HPLC Separation of Octopamine and Synephrine on Primesep 200 Column

HPLC Separation of Octopamine and Synephrine on Primesep 200 Column

Octopamine is an analog of norepinephrine in invertebrates that’s an important neurotransmitter and hormone. Synephrine is another molecule that has a similar structure to norepinephrine and is a very strong agonist of octopamine receptors in invertebrates. Both compounds can be baseline separated with an isocratic method in 30/70 Acetonitrile (ACN) and water mobile phase with a 30mM Ammonium Formate (AmFm) buffer at pH 3. UV detection 270nm.

 

Condition

Column Primesep 200, 4.6×150 mm, 5 µm, 100A
Mobile Phase MeCN/H2O – 30/70%
Buffer AmFm pH 3.0- 30 mM
Flow Rate 1.0 ml/min
Detection UV, 270 nm

 

Description

Class of Compounds
Drug, Acid, Hydrophilic, Ionizable,Neurotransmitter
Analyzing Compounds Octopamine, Synephrine

 

Application Column

Primesep 200

The Primesep family of mixed-mode columns offers a wide variety of stationary phases, boasting unprecedented selectivity in the separation of a broad array of chemical compounds across multiple applications. Corresponding Primesep guard columns, available with all stationary phases, do not require holders. SIELC provides a method development service available to all customers. Inquire about our specially-tailored custom LC-phases for specific separations.

Select options
Application Analytes:
Octopamine
Synephrine

Application Detection:
UV Detection
SIELC Technologies usually develops more than one method for each compound. Therefore, this particular method may not be the best available method from our portfolio for your specific application. Before you decide to implement this method in your research, please send us an email to research@sielc.com so we can ensure you get optimal results for your compound/s of interest.