Buffer Recommendation

 
Buffer
pH range UV range, nm

MS, ELSD

TFA 1.5-2.5 >210 +
Acetic acid 3.5-5.0 >220 ++
Formic acid 2.5-4.0 >220 ++
H2SO4 1.0-2.5 >195 +-
HClO4 1.5-2.5 >195
H3PO4 1.5-3.0 >195
TEA phosphate 1.8-3.1 >195
Sodium Phosphate 1.8-3.1 >195
    6.0-7.0 >195
Ammonium Phosphate 1.8-3.1 >195
TEA Formate 2.5-4.0 >220 +
TEA Acetate 3.5-5.0 >220 +
Ammonium Formate 2.5-4.0 >220 ++
Ammonium Acetate 3.5-5.0 >220 ++

 

Physical Properties of Acids Useful in HPLC Application

 
Name
Formula F.W. Density, g/ml Boiling point, oC UV range, nm Ion strength of 0.1% solution, mM pH of 0.1% solution
  Sulfuric acid H2SO4 98.08 1.840 high >195 20 2.0
  Perchloric acid HClO4 100.46 1.664 high >195 20 2.0
  Phosphoric acid H3PO4 98.00 1.685 high >195 15 2.1
  Trifluoroacetic acid (TFA) CF3COOH 115.03 1.493 75 >210 20 2.0
  Methanesulfonic acid CH3SO3H 96.11 1.481 high >195 20 2.0
Formic acid HCOOH 46.03 1.220 100 >220 2 3.0
Acetic acid CH3COOH 60.05 1.049 117 >220 0.2 4.0