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Obelisc N HPLC Columns

Obelisc N is a column which has very polar characteristics and works well for polar and charged analytes.

  • Obelisc N offers both positively and negatively charged groups to interact with positively or negatively charged analytes.
  • Multiple separation modes (NP, HILIC, IE).
  • In HILIC mode, Obelisc N offers different selectivity form other HILIC or bare silica columns.
  • Mass spec, ELSD, preparative and low UV (<220 nm) compatible, low concentration buffers.


Obelisc N columns are available in all standard dimensions
Column IDs: 22 mm, 10 mm, 4.6 mm, 3.2 mm, 2.1 mm, 1 mm, 0.5 mm, 0.25 mm
Column Lengths: 250 mm, 150 mm, 100 mm, 50 mm, 25 mm, 10 mm
Particles: 10 um, 5 um
Pores: 100 A


Obelisc N Application Examples


Comparison of Obelisc N, SeQuant® HILIC, and Bare Silica
Effect of both pH and Organic Content on a Separation of Sugars, Amino Acids, and Carboxylic Acids
Effect of Buffer on HPLC Separation of Buffers
HILIC Retention of 1,5-Naphthalenedisulfonic Acid on Obelisc N Mixed-Mode HILIC Columns
HILIC Separation of Aromatic Acids
HILIC Separation of Carboxylic Acids
HILIC Separation of Carboxymethylcysteine and Betaine
HILIC Separation of Common Preservatives - Citric Acid, Ascorbic Acid and EDTA
HILIC Separation of Epinephrine and Epinephrine Sulfonate
HILIC Separation of Polyethylene Glycol
HILIC Separation of Sugars on Obelisc N
HILIC Separation of Surfactant Triton X-100
HILIC Separation of Vitamins Group B
HPLC Analysis of TFA
HPLC Retention of Sulfuric Acid on Obelisc N Column
HPLC Separation of alpha-Aminobutyric, beta-Aminobutyric, and gamma-Aminobutyric acids on Obelisc N
HPLC Separation of Glyphosate Reaction Intermediates and Impurities
HPLC Separation of Nucleic Bases at pH 4 and 5 on Obelisc N
HPLC Separation of Polar and Hydrophobic Drugs on Obelisc R and N
HPLC Separation of Potassium, Perchlorate, Methanesulfonic, Chloride, Bromide, and Nitrate Ions on Obelisc N
HPLC Separation of Vitamin C, Vitamin Group B, and Related Impurities
Retention of Azide Ion on Obelisc N Column
Retention of N-Nitrosarcosine on Obelisc N Column
Retention of TFA on Atlantis and Obelisc N
Separation of Serotonin, Dopamine, and Related Compounds
Simultaneous Determination of Sodium and Sulfate Ions on Obelisc N Mixed-mode HILIC Column
Simultaneous Separation of Melamine and Cyanuric Acid
HPLC Analysis of Azide on Obelisc N Column 
HPLC Separation of Maleic Acid and Pentaerythritol 
HPLC Separation of Phosphorous and Phosphoric Acids 

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