Primesep 100

Primesep 100 is a reverse-phase analytical column with embedded acidic ion-pairing groups.

  • This is the most versatile column of the entire Primesep family for separation of a broad range of compounds.
  • Improves retention of basic compounds by cation-exchange mechanism.
  • Separates acids by ion-exclusion mechanism.
  • Retains neutral compounds by reverse-phase mechanism.
  • All mobile phases are LC-MS and preparative chromatography compatible.

Primesep 100 columns are available in all standard dimensions
Column IDs: 22 mm, 10 mm, 4.6 mm, 3.2 mm, 2.1 mm, 1 mm, 0.5 mm, 0.25 mm
Column Lengths: 250 mm, 150 mm, 100 mm, 50 mm, 25 mm, 10 mm
Particles: 10 um, 5 um
Pores: 100 A


Primesep 100 Application Examples

2,4-D Retention on Primesep 100 and Obelisc R

1-Naphthaleneacetic acid (NAA) Separation on Primesep and Obelisc Mixed-Mode Columns

16a-Hydroxyestradiol Separation on Mixed-Mode HPLC Columns

4-CPA Analysis on Obelisc and Primesep Mixed-Mode Columns

Amino Acids Analysis in Acid Gradient Condition

Amino Sugars Analysis in Ion Exchange and Reverse Mode

Analysis of Folic Acid and Related Impurities

Analysis of Nicotinic Acid in Nicotinamide

Complex Mixture of Acids, Bases, Amino Acids, and Neutral Compounds

Controlling Retention of 2,4-DB on Mixed-Mode HPLC Columns

Controlling Retention of Azinphos-ethyl on Primesep and Obelisc HPLC Columns

Dewetting Study in Aqueous Conditions

Dichlorprop (2,4-DP) Analysis on Primesep and Obelisc HPLC Columns

Diclobutrazol Separation on Primesep and Obelisc HPLC Columns

Effect of Buffer and Chemistry of Column Stationary Phase on Resolution of Neutral Compounds

Effect of Buffer Concentration on Resolution of Neutral Compounds (Prednisolone and D-Glucose Pentaacetate)

Effect of Concentration of Organic Modifier on Retention of Cation and Anions

Folpet Analysis on Primesep 100 and Obelisc R HPLC Columns

HPLC Analysis of 17a-Ethynylestradiol

HPLC Analysis of 2,4,5-T on Mixed-Mode Columns

HPLC Analysis of 5-Fluorouracil on Primesep Columns with LC/MS Compatible Mobile Phase

HPLC Analysis of Active Drug and Amino Acids in a Formulation

HPLC Analysis of Bentazon on Primesep and Obelisc Mixed-Mode Columns

HPLC Analysis of Calcium Ions in Maple Syrup

HPLC Analysis of Cheese Extract using Mixed-Mode Chromatography

HPLC Analysis of Citrulline and Arginine on Primesep Columns

HPLC Analysis of Dichloran on Mixed-Mode Columns

HPLC Analysis of Diphenamid on Primesep 100 and Obelisc R

HPLC Analysis of Fatty Acids with MS Compatible Conditions

HPLC Analysis of Fentin on Obelisc and Primesep Mixed-Mode Columns

HPLC Analysis of Inosine on Primesep 100 Column

HPLC Analysis of MCPA on Primesep and Obelisc Mixed-Mode Columns

HPLC Analysis of Motrin Cough & Cold Composition

HPLC Analysis of Piperazine on Primesep Columns

HPLC Application for Analysis of Sodium Succinate on Primesep 100

HPLC Application for Retention of Guanidine by Mixed-Mode Chromatography

HPLC Application for Simultaneous Separation of Amino Acids, Hydrophilic Acidic and Hydrophobic Neutral Compounds

HPLC Diazinon Analysis on Primesep 100 and Obelisc R columns

HPLC Method for Analysis of Fluconazole

HPLC Method for Determination of Lysine in Ibuprofen Lysine Composition

HPLC Retention Control of Antibiotics in Toothpaste

HPLC Retention of Aminopyrazole

HPLC Retention of Chlorpyrifos-methyl on Primesep 100 and Obelisc R Mixed-Mode Columns

HPLC Retention of Copper Ion on Primesep 100 Mixed-Mode Cation-Exchange Columns

HPLC Retention of Glucosamine

HPLC Retention of Pirimicarb on Primesep 100 and Obelisc R

HPLC Separation of 2-Naphthoxyacetic acid

HPLC Separation of 8 Generic Compounds on Primesep Columns

HPLC Separation of a Complex of Quinolone Antibiotics

HPLC Separation of Acidic, Basic and Zwitterionic Compounds on Special Columns for Retention of Polar Compounds

HPLC Separation of Albuterol and Amoxicillin

HPLC Separation of Amino Acids

HPLC Separation of Amino Acids in Supplements Capsules

HPLC Separation of Amino Acids in Supplements Composition in Mixed-Mode

HPLC Separation of Aminopyridines in Non-Aqueous Mobile Phase

HPLC Separation of Aromatic Sulfonamides and Hydrozine

HPLC Separation of Benzenesulfonamide and Sulfanilamide

HPLC Separation of Caffeine and Phenylephrine

HPLC Separation of Captan on Obelisc R and Primesep 100 Columns

HPLC Separation of Carbofuran on Obelisc R and Primesep 100 Columns

HPLC Separation of Carboxylic Acids

HPLC Separation of Catecholamines on Primesep 100 Column with Phosphate Buffers

HPLC Separation of Cidofovirs Mixture

HPLC Separation of Codeine, Oxycodone, and Hydrocodone

HPLC Separation of Components of Excedrin

HPLC Separation of DMSO and Glycine by Mixed-Mode Chromatography

HPLC Separation of DOPAC, Homovanillic Acid and Dopamine on the Primesep 100 Column

HPLC Separation of Epinephrine and Epinephrine Sulfonate

HPLC Separation of Estrone

HPLC Separation of Ethanolamines

HPLC Separation of GABA and GLU

HPLC Separation of Glutamic Acid and GABA

HPLC Separation of Glyphosate Production Intermediates

HPLC Separation of Guaifenesin and Terbutaline

HPLC Separation of Guanidine and Benzoic Acids on Primesep 100 Column

HPLC Separation of Hydrocortisone and Hydrocortisone’s Degradation Products

HPLC Separation of Impurities of Aminosugar

HPLC Separation of Isomers of Amino Salicylic Acid

HPLC Separation of Isomers of Aminobenzoic Acid

HPLC Separation of Metribuzin on Mixed-Mode HPLC Columns

HPLC Separation of Nabam on Mixed-Mode Columns

HPLC Separation of Phenylalanine, Glucosamine, and Tryptophan on Mixed-Mode Column

HPLC Separation of Phenylephrine with Tannic Acid Formulation

HPLC Separation of Phosphamidon on Mixed-Mode Columns

HPLC Separation of Phthalic Acids using Hydrogen Bonding

HPLC Separation of Pyridinecarboxylic Acids

HPLC Separation of Serine, a-Methylserine, GABA, and Methionine Mixture

HPLC Separation of Toluidine Isomers

HPLC Separation of Triclopyr

HPLC Separation of TRIS

Hydrophobic and Hydrophilic Compound Separation

Loading Study for Preparative Chromatography

MCPB Analysis Using Obelisc and Primesep Analytical HPLC Columns

Mecoprop Analysis on Primesep and Obelisc Mixed-Mode HPLC Columns

Nickel Contamination in Hydrogenation Products

Paraquat Diquat Separation in Non-aqueous Mobile Phase

Potassium Analysis in Organic Reaction Product

Retaining the Fungicide Carboxin on Primesep and Obelisc HPLC Columns

Retention Control of Bromacil on Primesep and Obelisc Mixed-Mode Columns

Retention of Aminolevulinic Acid in Mixed-Mode System

Retention of Monocrotophos on Primesep 100 and Obelisc R Columns

Retention of the Herbicide Dicamba on Mixed-Mode HPLC Columns

Reverse Elution Order in Separation of Alkali Cation

Reverse Mode vs Normal Mode: Separation of Aromatic Compounds

Separating Triadimefon from Impurities on Primesep and Obelisc HPLC Columns

Separation of Diacid: Ion Exclusion mode

Separation of Glyphosate by Cation Exchange and Anion Exchange

Separation of Guanidine

Separation of Isomers of Aminobenzoic Acid

Separation of Metal Ions on Primesep 100 Column

Separation of Oxytetracycline and Chlortetracycline in Mixed-Mode

Separation of Sodium, Potassium, Lithium, and Ammonium in Mixed-Mode HPLC

Separation of Steroids in Reverse and Normal Mode on Primesep 100 Column

Separation of Sulfonamides and Phenylhydrazine by Mixed-Mode HPLC

Separation of Sulphonium Ions in Polar Organic Mode

Separation of Thiabendazole on Mixed-Mode HPLC Columns

Separation of Zn and Mn on Primesep 100 Column

Simultaneous Separation of Cations and Anions

Terbufos Analysis on Primesep 100 and Obelisc R Columns

Zn and Na Quantitation in Drug Substances