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Primesep 100 HPLC Columns

Primesep 100 is a reverse-phase analytical column with embedded acidic ion-pairing groups.

  • This is the most versatile column of the entire Primesep family for separation of a broad range of compounds.
  • Improves retention of basic compounds by cation-exchange mechanism.
  • Separates acids by ion-exclusion mechanism.
  • Retains neutral compounds by reverse-phase mechanism.
  • All mobile phases are LC-MS and preparative chromatography compatible.


Primesep 100 columns are available in all standard dimensions
Column IDs: 22 mm, 10 mm, 4.6 mm, 3.2 mm, 2.1 mm, 1 mm, 0.5 mm, 0.25 mm
Column Lengths: 250 mm, 150 mm, 100 mm, 50 mm, 25 mm, 10 mm
Particles: 10 um, 5 um
Pores: 100 A


Primesep 100 Application Examples


Amino Acids Analysis in Acid Gradient Condition
Amino Acids in Supplements Capsules
Amino Sugars Analysis in Ion Exchange and Reverse Mode
Complex Mixture of Acids, Bases, Amino Acids, and Neutral Compounds
Dewetting Study in Aqueous Conditions
Effect of Buffer and Chemistry of Column Stationary Phase on Resolution of Neutral Compounds
Effect of Concentration of Organic Modifier on Retention of Cation and Anions
HPLC Analysis of 5-Fluorouracil on Primesep Columns with LC/MS Compatible Mobile Phase
HPLC Analysis of Active Drug and Amino Acids in a Formulation
HPLC Analysis of Cheese Extract using Mixed-Mode Chromatography
HPLC Analysis of Citrulline and Arginine on Primesep Columns
HPLC Analysis of Fatty Acids with MS Compatible Conditions
HPLC Analysis of Motrin Cough & Cold Composition
HPLC Application for Analysis of Sodium Succinate on Primesep 100
HPLC Application for Retention of Guanidine by Mixed-Mode Chromatography
HPLC Application for Simultaneous Separation of Amino Acids, Hydrophilic Acidic and Hydrophobic Neutral Compounds
HPLC Method for Determination of Lysine in Ibuprofen Lysine Composition
HPLC Retention of Aminopyrazole
HPLC Retention of Copper Ion on Primesep 100 Mixed-Mode Cation-Exchange Columns
HPLC Retention of Glucosamine
HPLC Separation of 8 Generic Compounds on Primesep Columns
HPLC Separation of Acidic, Basic and Zwitterionic Compounds on Special Columns for Retention of Polar Compounds
HPLC Separation of Amino Acids
HPLC Separation of Amino Acids in Supplements Composition in Mixed-Mode
HPLC Separation of Aromatic Sulfonamides and Hydrozine
HPLC Separation of Benzenesulfonamide and Sulfanilamide
HPLC Separation of Caffeine and Phenylephrine
HPLC Separation of Carboxylic Acids
HPLC Separation of Codeine, Oxycodone, and Hydrocodone
HPLC Separation of Components of Excedrin
HPLC Separation of DMSO and Glycine by Mixed-Mode Chromatography
HPLC Separation of Epinephrine and Epinephrine Sulfonate
HPLC Separation of GABA and GLU
HPLC Separation of Glyphosate Production Intermediates
HPLC Separation of Guaifenesin and Terbutaline
HPLC Separation of Guanidine and Benzoic Acids on Primesep 100 Column
HPLC Separation of Hydrocortisone and Hydrocortisone's Degradation Products
HPLC Separation of Impurities of Aminosugar
HPLC Separation of Isomers of Amino Salicylic Acid
HPLC Separation of Isomers of Aminobenzoic Acid
HPLC Separation of Phenylalanine, Glucosamine, and Tryptophan on Mixed-Mode Column
HPLC Separation of Phenylephrine with Tannic Acid Formulation
HPLC Separation of Pyridinecarboxylic Acids
HPLC Separation of Serine, a-Methylserine, GABA, and Methionine Mixture
HPLC Separation of Toluidine Isomers
HPLC Separation of TRIS
Hydrophobic and Hydrophilic Compound Separation
Loading Study for Preparative Chromatography
Neutral, Base, and Acid on Primesep 100 Column
Nickel Contamination in Hydrogenation Products
Potassium Analysis in Organic Reaction Product
Retention of Aminolevulinic Acid in Mixed-Mode System
Reverse Mode vs Normal Mode: Separation of Aromatic Compounds
Separation of Albuterol and Amoxicillin
Separation of Cidofovirs Mixture (2)
Separation of Diacid: Ion Exclusion mode
Separation of Ethanolamines
Separation of Glyphosate by Cation Exchange and Anion Exchange
Separation of Guanidine
Separation of Metal Ions on Primesep 100 Column
Separation of Sodium, Potassium, Lithium, and Ammonium in Mixed-Mode HPLC
Separation of Steroids in Reverse and Normal Mode on Primesep 100 Column
Separation of Sulfonamides and Phenylhydrazine by Mixed-Mode HPLC
Separation of Sulphonium Ions in Polar Organic Mode
Separation of Zn and Mn on Primesep 100 Column
Simultaneous Separation of Cations and Anions
Zn and Na Quantitation in Drug Substances
Analysis of Calcium Ions in Maple Syrup 
HPLC Analysis of Inosine on Primesep 100 Column 
HPLC Analysis of Piperazine on Primesep Columns 
HPLC Separation of DOPAC, Homovanillic Acid in Dopamine on the Primesep 100 Column

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