Products  →  Primesep  →  Primesep 200

Primesep 200 HPLC Columns

Primesep 200 is a reverse-phase analytical column with embedded weak acidic ion-pairing groups.

  • Improves retention of strong basic compounds by cation-exchange mechanism.
  • Retains neutral compounds by reverse-phase mechanism.
  • Provides an additional interaction with polar molecules offering separation of isomers and structurally-related compounds.
  • All mobile phases are LC-MS and preparative chromatography compatible.


Primesep 200 columns are available in all standard dimensions
Column IDs: 22 mm, 10 mm, 4.6 mm, 3.2 mm, 2.1 mm, 1 mm, 0.5 mm, 0.25 mm
Column Lengths: 250 mm, 150 mm, 100 mm, 50 mm, 25 mm, 10 mm
Particles: 10 um, 5 um
Pores: 100 A

 


Primesep 200 Application Examples


Bufferless Ion Separation (BLIS™) Chromatography of Amino Acids (1)
Bufferless Ion Separation (BLIS™) Chromatography of Amino Acids (2)
Catecholamine Pathway
Effect of Buffer and Chemistry of Column Stationary Phase on Resolution of Neutral Compounds
HILIC Separation of Hydroxypiperidine and Impurities
HPLC Analysis of Apple Extract using Mixed-Mode Chromatography
HPLC Analysis of Citrulline and Arginine on Primesep Columns
HPLC Application for Determination of Melamine in Baby Formula
HPLC Application for Separation of Nucleotide Bases
HPLC Retention of Aminopyrazole
HPLC Separation of 8 Generic Compounds on Primesep Columns
HPLC Separation of Acidic, Basic and Zwitterionic Compounds on Special Columns for Retention of Polar Compounds
HPLC Separation of Acidic, Basic, and Neutral Compounds
HPLC Separation of Active Compounds in Drug Formulation
HPLC Separation of Caffeine and Phenylephrine
HPLC Separation of Citrulline and Arginine
HPLC Separation of Codeine, Oxycodone, and Hydrocodone
HPLC Separation of Compounds of Catecholamine Pathway
HPLC Separation of Creatine, Creatinine and Histidine
HPLC Separation of Creatine, Creatinine and Histidine with MS Conditions
HPLC Separation of Epinephrine and Related Impurities
HPLC Separation of Guaifenesin and Terbutaline
HPLC Separation of Histamine and Phenethylamine
HPLC Separation of Nucleobases (2)
HPLC Separation of Polar Compounds
HPLC Separation of Tetracyclines
HPLC Separation of Toluidine Isomers
Mixed-Mode HPLC Separation of Tertiary Amines on Primesep 200 Column
Neurotransmitters
Neutral, Base, and Acid on Primesep 200 Column
Retention Characteristics of Betaine in Mixed-Mode HPLC
Separation of Catecholamines
Separation of Cotinine Metabolites
Separation of Deoxyribo Nucleic Acid
Separation of Diacid Hydrophobic and Ion Exclusion Modes
Separation of Epinephrine Norepinephrine
Separation of Gentamicins
Separation of Nucleic Bases
Separation of Polyamines
Separation of Simple Tertiary Amines
Separation of Sodium, Tetramethylammonium and Tetrabutylammonium on Primesep 200 Column
HPLC Analysis of Bendamustine and Related Impurities
HPLC Analysis of Piperazine on Primesep Columns
HPLC Separation of 1-Methylhistidine and 3-Methylhistidine on Primesep 200 
HPLC Separation of DOPAC, Homovanillic Acid in Dopamine on the Primesep 200 Column 
HPLC Separation of Succinylcholine and Choline Using a Primesep 200 Column 

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