Primesep 500

Primesep 500 is a reverse-phase analytical column with embedded acidic ion-pairing groups.

  • Primesep 500 has an embedded carboxilic acid with a pKa of about 5.
  • Improves retention of basic compounds by cation-exchange mechanism at pH > 5.
  • Separates acids by ion-exclusion mechanism at a mobile phase pH > 5.
  • Retains basic compounds, as any RP column, at pH < 5
  • Retains neutral compounds by reverse-phase mechanism.
  • All mobile phases are LC-MS and preparative chromatography compatible.

Primesep 500 columns are available in all standard dimensions
Column IDs: 22 mm, 10 mm, 4.6 mm, 3.2 mm, 2.1 mm, 1 mm, 0.5 mm, 0.25 mm
Column Lengths: 250 mm, 150 mm, 100 mm, 50 mm, 25 mm, 10 mm
Particles: 10 um, 5 um
Pores: 100 A


Primesep 500 Application Examples

HPLC Separation of Alkyl Sulfonic Acids

HPLC Separation of Glucosamine from Chondroitin Sulfate