Primesep A

Primesep A is a reverse-phase analytical column with embedded strong acidic ion-pairing groups.

  • Improves retention of weak basic compounds by cation-exchange mechanism.
  • Separates acids by ion-exclusion mechanism.
  • Retains neutral compounds by reverse-phase mechanism.
  • All mobile phases are LC-MS and preparative chromatography compatible.

Primesep A columns are available in all standard dimensions
Column IDs: 22 mm, 10 mm, 4.6 mm, 3.2 mm, 2.1 mm, 1 mm, 0.5 mm, 0.25 mm
Column Lengths: 250 mm, 150 mm, 100 mm, 50 mm, 25 mm, 10 mm
Particles: 10 um, 5 um
Pores: 100 A


Primesep A Application Examples

HPLC Retention of Aminopyrazole

HPLC Separation of Taurine

Mixed-Mode Retention and Separation of Ammonia, Primary and Secondary Amines on Primesep A Column

Neutral, Base, and Acid on Primesep A Column

Separation of Simple Amines and Ammonia

TFA Concentration Effect on Retention of Tricine