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Primesep B2 HPLC Columns

Primesep B2 is a reverse-phase analytical column with embedded basic ion-pairing groups.

  • Improves retention of acidic compounds by anion-exchange mechanism.
  • Separates bases by ion-exclusion mechanism.
  • Retains neutral compounds by reverse-phase mechanism.
  • All mobile phases are LC-MS and preparative chromatography compatible (working pH range from 1.5 to 7).


Primesep B2 columns are available in all standard dimensions
Column IDs: 22 mm, 10 mm, 4.6 mm, 3.2 mm, 2.1 mm, 1 mm, 0.5 mm, 0.25 mm
Column Lengths: 250 mm, 150 mm, 100 mm, 50 mm, 25 mm, 10 mm
Particles: 10 um, 5 um
Pores: 100 A


Primesep B2 Application Examples


Effect of Buffer and Chemistry of Column Stationary Phase on Resolution of Neutral Compounds
HPLC Analysis of Gemfibrozil and Ibuprofen Using Mixed-Mode Chromatography
HPLC Analysis of Sulfate and Phosphate Ions on Primesep Columns
HPLC Analysis of Valproic Acid using Mixed-Mode Chromatography
HPLC Retention of Diazacon on Primesep B2 Mixed-mode Anion-Exchange Column
HPLC Retention of Fentin Acetate
HPLC Separation of Carboxylic Acids
HPLC Separation of Glyphosate Production Intermediates
HPLC Separation of Lactic Acid and Its Oligomers
HPLC Separation of m-Cresol Purple and Related Impurities
HPLC Separation of Nitroprusside and its Metabolites
HPLC Separation of Parabens and Benzoic Acid
HPLC Separation of Sulfate and Phosphate Ions on Mixed-Mode HPLC
Improved Peak Shape of a Basic Compound on Primesep B2 Column
Separation and Retention of Dextromethorphan Bromide in Mixed-Mode HPLC: Buffer Concentration Effect
Separation and Retention of Vitamins in Mixed-Mode HPLC
Separation of Chlorate and Bromate Ions
Separation of Two Vitamins: Different Polarity - Isocratic Methods
Separation Study of Fentin Acetate in Mixed-Mode HPLC

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