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Primesep C HPLC Columns

Primesep C is a reverse-phase analytical column with embedded complex-forming groups.

  • Improves retention of positively charged compounds by forming a host-guest complex.
  • Retains neutral compounds by reverse-phase mechanism.
  • All mobile phases are LC-MS and preparative chromatography compatible.


Primesep C columns are available in all standard dimensions
Column IDs: 22 mm, 10 mm, 4.6 mm, 3.2 mm, 2.1 mm, 1 mm, 0.5 mm, 0.25 mm
Column Lengths: 250 mm, 150 mm, 100 mm, 50 mm, 25 mm, 10 mm
Particles: 10 um, 5 um
Pores: 100 A



Primesep C Application Examples

Bufferless Ion Separation (BLIS™) Chromatography of Aspartame: Organic Modifier Effect
Complex of Cations on Primesep C is Effected by Organic Modifier
Control of Cation Exchange Properties by pH of Primesep C Column
Effect of Buffer and Chemistry of Column Stationary Phase on Resolution of Neutral Compounds
Effect of pH on Retention of Basic Compounds on Primesep C Columns
HPLC Analysis of Alginic Acid in Heartburn Tablets
HPLC Retention of Fentin Acetate
HPLC Retention of Obidoxime
HPLC Retention of Trimipramine
HPLC Separation of Acetaminophen, Phenylephrine, and Diphenhydramine
HPLC Separation of Antibiotics in Fish Production
HPLC Separation of Common Antibiotics in Fish Farming
HPLC Separation of Drugs
HPLC Separation of Explosives
HPLC Separation of Histamine and Phenethylamine
HPLC Separation of NyQuil Active
HPLC Separation of Polar Drugs with MS-compatible Method
HPLC Separation of Surfynol 420
Primesep C Column Retention Control
Reverse Elution Order in Separation of Alkali Cation
Separation of Acetylcholines
Separation of Alginic Acid and Related Products
Separation of Isomers of Aminobutyric Acids
Separation of NyQuil Active Ingredients on Primesep C
Separation of Quaternary Amines
Separation of Vitamin B
Separation Study of Fentin Acetate in Mixed-Mode HPLC
Separation Study of Zemuron Active Ingredient in Mixed-Mode HPLC
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