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Primesep D HPLC Columns

Primesep D is a reverse-phase analytical column with embedded basic ion-pairing groups specifically design for direct plasma analysis.

  • Does not retain high-molecular weight plasma components.
  • Retains hydrophobic and acidic components.
  • All mobile phases are LC-MS and preparative chromatography compatible (working pH range from 1.5 to 4).


Primesep D columns are available in all standard dimensions
Column IDs: 22 mm, 10 mm, 4.6 mm, 3.2 mm, 2.1 mm, 1 mm, 0.5 mm, 0.25 mm
Column Lengths: 250 mm, 150 mm, 100 mm, 50 mm, 25 mm, 10 mm
Particles: 10 um, 5 um
Pores: 100 A


Primesep D Application Examples


Effect of Buffer and Chemistry of Column Stationary Phase on Resolution of Neutral Compounds
HPLC Analysis of Apple Extract using Mixed-Mode Chromatography
HPLC Analysis of Basic Drugs and Acidic Counter-Ions by Mixed-Mode Chromatography
HPLC Analysis of Cheese Extract using Mixed-Mode Chromatography
HPLC Analysis of Drugs in Saliva
HPLC Analysis of Gemfibrozil and Ibuprofen Using Mixed-Mode Chromatography
HPLC Analysis of Polysorbate in Mixture with Amino Acids and Sugar
HPLC Analysis of Sulfate and Phosphate Ions on Primesep Columns
HPLC Application for Analysis of EDTA
HPLC Retention of Ethylglucuronide
HPLC Retention of Fentin Acetate
HPLC Retention of N-Cyclohexylsulfamic Acid
HPLC Retention of Polysorbate on Primesep D Column
HPLC Separation of 3-Nitrophthalic and 4-Nitrophthalic Acids on Primesep D HPLC Column
HPLC Separation of Aminobiphenyls
HPLC Separation of Benzenesulfonamide and Sulfanilamide
HPLC Separation of Benzenesulfonic and p-Toluenesulfonic Acids
HPLC Separation of Cetylpyridinium
HPLC Separation of Chloride and Nitrate Ions
HPLC Separation of Dequalinium and Benzonitrile on Different Columns
HPLC Separation of Dihydroxybenzoic Acid
HPLC Separation of Dyes
HPLC Separation of Hydrocortisone and Hydrocortisone's Degradation Products
HPLC Separation of Organics Acids
HPLC Separation of Succinic and Methylmalonic Acids
HPLC Separation of Sulfate and Phosphate Ions on Mixed-Mode HPLC
HPLC Separation of Surfactants
HPLC Separation of Taurine
HPLC Separation of Vanilmandelic, Hydroxyindoleacetic, and Homovanillic Acids
HPLC Separation of Vitamin C and Vitamin B2
Separation of Cidofovirs Mixture (2)
Separation of Vitamins in Mixed-Mode HPLC
Separation Study of Fentin Acetate in Mixed-Mode HPLC
HPLC Seperation of Organic Acids 
HPLC Analysis of Bendamustine and Related Impurities 
HPLC Separation of Phosphorous and Phosphoric Acids 

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