Primesep D

Primesep D is a reverse-phase analytical column with embedded basic ion-pairing groups specifically design for direct plasma analysis.

  • Does not retain high-molecular weight plasma components.
  • Retains hydrophobic and acidic components.
  • All mobile phases are LC-MS and preparative chromatography compatible (working pH range from 1.5 to 4).

Primesep D columns are available in all standard dimensions
Column IDs: 22 mm, 10 mm, 4.6 mm, 3.2 mm, 2.1 mm, 1 mm, 0.5 mm, 0.25 mm
Column Lengths: 250 mm, 150 mm, 100 mm, 50 mm, 25 mm, 10 mm
Particles: 10 um, 5 um
Pores: 100 A


Primesep D Application Examples

Analysis of Antibiotic Josamycin and Related Impurities in Mixed-Mode Chromatography

Analysis of Antibiotic Marbofloxacin and Related Impurities in Mixed-Mode Chromatography

Analysis of Antibiotic Norfloxacin and Related Impurities in Mixed-Mode Chromatography

Analysis of Lactic Acid on Primesep D Column

Analysis of Methylene Blue Purity in Reversed-Phase Cation-Exchange and Reversed-Phase Cation-Exclusion Modes

Effect of Buffer and Chemistry of Column Stationary Phase on Resolution of Neutral Compounds

HPLC Analysis of Apple Extract using Mixed-Mode Chromatography

HPLC Analysis of Basic Drugs and Acidic Counter-Ions by Mixed-Mode Chromatography

HPLC Analysis of Bendamustine and Related Impurities

HPLC Analysis of Drugs in Saliva

HPLC Analysis of Gemfibrozil and Ibuprofen Using Mixed-Mode Chromatography

HPLC Analysis of Polysorbate in Mixture with Amino Acids and Sugar

HPLC Analysis of Sulfate and Phosphate Ions on Primesep Columns

HPLC Application for Analysis of EDTA

HPLC Retention of Ethylglucuronide

HPLC Retention of Fentin Acetate

HPLC Retention of N-Cyclohexylsulfamic Acid

HPLC Retention of Polysorbate on Primesep D Column

HPLC Separation of 3-Nitrophthalic and 4-Nitrophthalic Acids on Primesep D HPLC Column

HPLC Separation of Aminobiphenyls

HPLC Separation of Antibiotics of Group in Mixed-Mode Chromatography

HPLC Separation of Benzalkonium Chloride BCT 8358 on Primesep D column

HPLC Separation of Benzenesulfonamide and Sulfanilamide

HPLC Separation of Benzenesulfonic and p-Toluenesulfonic Acids

HPLC Separation of Cetylpyridinium

HPLC Separation of Chloride and Nitrate Ions

HPLC Separation of Cidofovirs Mixture

HPLC Separation of Dequalinium and Benzonitrile on Different Columns

HPLC Separation of Dihydroxybenzoic Acid

HPLC Separation of Dyes

HPLC Separation of Hydrocortisone and Hydrocortisone’s Degradation Products

HPLC Separation of Organic Acids

HPLC Separation of Organics Acids

HPLC Separation of Phosphorous and Phosphoric Acids

HPLC Separation of Succinic and Methylmalonic Acids

HPLC Separation of Sulfate and Phosphate Ions on Mixed-Mode HPLC

HPLC Separation of Surfactants

HPLC Separation of Taurine

HPLC Separation of TRIS

HPLC Separation of Vanilmandelic, Hydroxyindoleacetic, and Homovanillic Acids

HPLC Separation of Vitamin C and Vitamin B2

Retention of Sialic Acid on Primesep D Column

Separation of Bisphenol A and Bisphenol A Glucoronide

Separation of Chlorate, Perchlorate, and Phosphonate Ions

Separation of Compounds in TCA Cycle on Primesep D Column

Separation of Phthalic Acids and Related Impurities

Separation of Vitamins in Mixed-Mode HPLC

Separation Study of Fentin Acetate in Mixed-Mode HPLC