Primesep SB

Primesep SB is a reverse-phase analytical column with strong embedded basic ion-pairing groups.

  • Improves retention of acidic compounds by anion-exchange mechanism.
  • Separates bases by ion-exclusion mechanism.
  • Retains neutral compounds by reverse-phase mechanism.
  • All mobile phases are LC-MS and preparative chromatography compatible (working pH range from 1.5 to 5).

Primesep SB columns are available in all standard dimensions
Column IDs: 22 mm, 10 mm, 4.6 mm, 3.2 mm, 2.1 mm, 1 mm, 0.5 mm, 0.25 mm
Column Lengths: 250 mm, 150 mm, 100 mm, 50 mm, 25 mm, 10 mm
Particles: 10 um, 5 um
Pores: 100 A


Primsep SB Application Examples

HPLC Analysis of ACE Serum Active Ingredients

HPLC Analysis of Hop Extract (Beer Brewing)

HPLC Analysis of L-Cysteine-S-Sulfate on Primesep SB Column

HPLC Retention of EDTA in Sulfated CD

HPLC Retention of Ethylglucuronide

HPLC Retention of Sulfamic Acid on Primesep SB Mixed-Mode Column

HPLC Separation of 2-HEP and 2-HPP in the Presence of Methylcobalamin and Amino Acids

HPLC Separation of 5-Methyltetrahydrofolic Acid and Degradation Products

HPLC Separation of Alendronic, Ibandronic, Etidronic Acids

HPLC Separation of Ascorbic and Dehydroascorbic Acids

HPLC Separation of Epinephrine and Epinephrine Sulfonate

HPLC Separation of Methylphosphonic Acid and Related Products

HPLC Separation of Sodium, Chloride, Bromide, and Iodine

HPLC Separation of Sugar Phosphates

Separation of Nine Nucleotides by Mixed-Mode Chromatography

Separation of Nucleotide Monophosphates