1. Use the following chart to select a column based on the analyte’s properties
2. Select the column length based on sample complexity
- 150-250 mm for proteomics, protein digests, and complex samples
- 50-150 mm for synthetic analysis and purification
3. Select the column i.d. based on sample loading and sensitivity needs
4. Mobile Phase should be pH 2-4 with:
5. Organic Modifier should be Acetonitrile
6. Ionic strength, pH, and organic modifier gradients can be used to optimize the separation
|
We recommend selecting a stationary phase with pore size 100 Å for Promix SP; pore size 100 or 300 Å for Promix AP; pore size 300 or 800 Å for Promix MP and pore size 800 Å or none porous for Promix LP.
|