Column Selection

1. Use the following chart to select a column based on the analyte's properties

2. Select the column length based on sample complexity

  • 150-250 mm for proteomics, protein digests, and complex samples
  • 50-150 mm for synthetic analysis and purification

3. Select the column i.d. based on sample loading and sensitivity needs

4. Mobile Phase should be pH 2-4 with:

  • TFA (0.05-0.3%)
  • Formic Acid (0.1-0.9%)
  • Ammonium Acetate or Ammonium Formate

5. Organic Modifier should be Acetonitrile

6. Ionic strength, pH, and organic modifier gradients can be used to optimize the separation

We recommend selecting a stationary phase with pore size 100 Å for Promix SP; pore size 100 or 300 Å for Promix AP; pore size 300 or 800 Å for Promix MP and pore size 800 Å or none porous for Promix LP.