HPLC Separation of Organic Acids Glucuronic Acid, Methanesulfonic Acid, and Butanesulfonic Acid on Primesep SB Column

The organic acids glucuronic acid, methanesulfonic acid, and butanesulfonic acid were separated using Primesep SB mixed-mode column. Primesep SB is a reverse-phase column with embedded basic ion-pairing groups.

 

Condition

Column Primesep SB, 4.6×50 mm, 5 µm, 100A
Mobile Phase MeCN/H2O – 30/70%
Buffer Gradient AmFm pH 3.0- 2-50 mM, 3 min, 3 min hold
Flow Rate 1.0 ml/min
Detection CAD

 

Description

Class of Compounds
Acid, Hydrophilic, Ionizable
Analyzing Compounds Glucuronic acid, Methanesulfonic acid, Butanesulfonic acid

 

Application Column

Primesep SB

The Primesep family of mixed-mode columns offers a wide variety of stationary phases, boasting unprecedented selectivity in the separation of a broad array of chemical compounds across multiple applications. Corresponding Primesep guard columns, available with all stationary phases, do not require holders. SIELC provides a method development service available to all customers. Inquire about our specially-tailored custom LC-phases for specific separations.

Select options
Application Analytes:
Benzenesulfonic Acid
Butanesulfonic acid
Glucuronic acid
Methanesulfonic Acid

Application Detection:
ELSD Detection
UV Detection
SIELC Technologies usually develops more than one method for each compound. Therefore, this particular method may not be the best available method from our portfolio for your specific application. Before you decide to implement this method in your research, please send us an email to research@sielc.com so we can ensure you get optimal results for your compound/s of interest.