Allantoin

Allantoin structural formula

CAS Number97-59-6
Molecular FormulaC4H6N4O3
Molecular Weight158.118
InChI KeyPOJWUDADGALRAB-UHFFFAOYSA-N
LogP-2.23
Synonyms
  • Allantoin
  • N-(2,5-Dioxoimidazolidin-4-yl)urea
  • Urea, N-(2,5-dioxo-4-imidazolidinyl)-
  • 97-59-6
  • 5-25-15-00338
  • Urea, (2,5-dioxo-4-imidazolidinyl)-
  • (.+-.)-Allantoin
  • (2,5-Dioxo-4-imidazolidinyl)urea
  • 5-Ureidohydantoin
  • alantoina
  • Allantoine
  • Allantol
  • Cordianine
  • DL-Allantoin
  • Glyoxyldiureid
  • GLYOXYLDIUREIDE
  • Glyoxylic diureide
  • NSC 7606
  • Psoralon
  • Sebical
  • Septalan
  • Urea, N-(2,5-dioxo-4-imidazolidinyl)-
  • BRN 0102364
  • Caswell No. 024
  • EINECS 202-592-8
  • EPA Pesticide Chemical Code 085701
  • Hydantoin, 5-ureido-
  • Uniderm A
  • 5-Ureido-2,4-imidazolidindion
  • Cutemol emollient
  • UNII-344S277G0Z
  • Herpecin L
  • (2,5-diketoimidazolidin-4-yl)urea
  • (2,5-dioxoimidazolidin-4-yl)urea
  • (S)-allantoin
  • 2,5-Dioxo-4-imidazolidinyl-urea
  • 4-ureido-2,5-imidazolidinedione
  • 5-Ureido-2,4-imidazolidindione
  • 5-Ureido-Hydantoin
  • 5-Ureidohydrantoin
  • AVC/Dienestrolcream
  • Alantan
  • Alloxantin
  • D00121
  • Fancol TOIN
  • N-(2,5-Dioxo-4-imidazolidinyl)urea
  • 37305-69-4
  • 5377-33-3
  • 58308-55-7

Applications:

HPLC Determination of Allantoin on SHARC 1 Column

February 1, 2021

HPLC Method for Allantoin on SHARC 1 by SIELC Technologies

High Performance Liquid Chromatography (HPLC) Method for Analysis of Allantoin.

Allantoin is a 5-ureidohydantoin or glyoxyldiureide with the chemical formula C4H6N4O3. It is s metabolic intermediate that is found in plants like chamomile, wheat sprouts, tobacco seed, sugar beet and comfrey. It is frequently used in cosmetics and hygiene products. 

Allantoin can be retained using HPLC on SHARC 1 column, which uses hydrogen-bonding as a separation mechanism with isocratic method by using a mobile phase of acetonitrile (ACN) and methanol (MeOH).  Allantoin can be monitored by low UV (210 nm), ELSD, CAD  or LC/MS.

 

Condition
Column SHARC 1, 4.6 x 150 mm, 5 µm, 100 A, dual ended
Mobile Phase MeCN/MeOH – 90/10%
Buffer No
Flow Rate 1.0 ml/min
Detection UV 210 nm

 

Description
Class of Compounds
 Hydrophilic, Amide, Amine
Analyzing Compounds Allantoin

 

Application Column

SHARC 1

Column Diameter: 4.6 mm
Column Length: 150 mm
Particle Size: 5 µm
Pore Size: 100 A
Column options: dual ended

Add to cart
Application Analytes:
Allantoin
Application Detection:
UV Detection
SIELC Technologies usually develops more than one method for each compound. Therefore, this particular method may not be the best available method from our portfolio for your specific application. Before you decide to implement this method in your research, please send us an email to research@sielc.com so we can ensure you get optimal results for your compound/s of interest.

HPLC Method for Analysis of Allantoin on Primesep 100 Column

May 3, 2018

HPLC Method for Allantoin on Primesep 100 by SIELC Technologies

High Performance Liquid Chromatography (HPLC) Method for Analysis of Allantoin

Allantoin is found in plants such as wheat sprouts, chamomile, sugar beet, and comfrey. It is used as a moisturizer, which is, as the name suggest, used to moisturize the skin and to treat or prevent skin irritations and dry skin. Primesep 100, a reverse-phase column, contains embedded acidic ionizable groups and can retain Allantoine. The method is LC/MS and UV compatible and can be used as a general approach for analyzing similar compounds.

ColumnPrimesep 100, 4.6 x 150 mm, 3 µm, 100 A, dual ended
Mobile PhaseMeCN/H2O – 10/90%
BufferTFA- 0.1%
Flow Rate1.0ml/min
DetectionUV, 210 nm
Class of Compounds
Plant-based compound
Analyzing CompoundsAllantoin

Application Column

Primesep 100

Column Diameter: 4.6 mm
Column Length: 150 mm
Particle Size: 3 µm
Pore Size: 100 A
Column options: dual ended

Add to cart
Application Analytes:
Allantoin
Application Detection:
UV Detection
SIELC Technologies usually develops more than one method for each compound. Therefore, this particular method may not be the best available method from our portfolio for your specific application. Before you decide to implement this method in your research, please send us an email to research@sielc.com so we can ensure you get optimal results for your compound/s of interest.