HPLC Application: Effect of Acid on Separation of Xanthines

Separation of Effect of Acid on Separation of Xanthines

Application Notes:

Primesep B2 separates xanthines such as theobromine, dimethylxanthine, theophylline by a combination of reversed-phase and ion-exchange mechanisms. The anion-exchange properties of the column allow retention of hydrophilic xanthines, and the reversed-phase properties retain hydrophobic compounds. The HPLC separation uses a mobile phase of water, acetonitrile (MeCN, ACN) and either trifluoroacetic acid (TFA), perchloric acid (HClO4), or sulfuric acid (H2SO4) and UV detection.

Application Columns:

Primesep^® B is a mixed-mode HPLC column with general hydrophobic and ion-exchange properties. The Primesep B stationary phase combines a hydrophobic chain and a strongly basic functional group as an anion exchanger recommended for mobile phase pH's of 1.5 to 4. Primesep B separates bases by an ion-exclusion mechanism. The basic functional group acts as an embedded ion-pairing reagent. Recommended buffers for Primesep columns are trifluoroacetic acid (TFA), phosphoric acid (H3PO4), perchloric acid (HClO4), and formic acid. Methods can be developed on Primesep columns that are fully compatible with detectors such as UV, RI, conductivity, fluorescence, evaporative light scattering (ELSD) as well as mass spectrometers (LC/MS) and preparative chromatography (prep HPLC) systems.

Application Analytes:

Application Detection:

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