HPLC Application: Dewetting Study in Aqueous Conditions

Separation of Dewetting Study in Aqueous Conditions

Application Notes:

Most reversed-phase HPLC columns, such as C18, are incompatible with 100% aqueous mobile phases due to dewetting (collapse) of the stationary phase. Primesep columns combine reversed-phase with polar functional groups which eliminate the problems of dewetting. The Primesep 100 column in this application with an acetonitrile (MeCN, ACN) shows a retention time of 2.5 minutes. In 100% water, the retention time is 3 minutes even after sitting 24 hours in the water mobile phase. On a C18 phase, the analyte retention would have been lost and elution at the void (1.5 minutes) would have resulted.

Application Columns:

Primesep^® 100 is a mixed-mode HPLC column with general hydrophobic and ion-exchange properties. The Primesep 100 stationary phase combines a hydrophobic chain and an acidic functional group as a cation exchanger. Acids, bases, zwitterions and neutrals can be retained and separated by Primesep 100 columns. Primesep 100 retains hydrophobic compounds by reversed-phase retention alone or a combination of reversed-phase and ion-exchange retention. Primesep 100 separates underivatized amino acids and amines by combining reversed-phase and ion-exchange mechanisms. In this case the acidic functional group acts as an embedded ion-pairing reagent. Organic acids are separated by reversed phase and ion exclusion. Inorganic cations can be separated by cation exchange. Methods can be developed on Primesep columns that are fully compatible with detectors such as UV, RI, conductivity, fluorescence, evaporative light scattering (ELSD) as well as mass spectrometers (LC/MS) and preparative chromatography (prep HPLC) systems.

Application Analytes:

Benzoquinone

Application Detection:

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