HPLC Application: Amino Sugars Analysis in Ion Exchange and Reverse Mode

Separation of Amino Sugars Analysis in Ion Exchange and Reverse Mode

Application Notes:

Primesep 100 retains an amino sugar by a combination of cation exchange and reversed phase. Columns with reversed-phase functionality alone are unable to retain this amino sugar. The polar interactions of the Primesep 100 column together with the reversed-phase interactions retain the amino sugar and resolve it from its many impurities. The separation method uses a mobile phase mixture of water, acetonitrile (MeCN, ACN) and sulfuric acid (H2SO4) with UV detection at 205 nm.

Application Columns:

Primesep^® 100 is a mixed-mode HPLC column with general hydrophobic and ion-exchange properties. The Primesep 100 stationary phase combines a hydrophobic chain and an acidic functional group as a cation exchanger. Acids, bases, zwitterions and neutrals can be retained and separated by Primesep 100 columns. Primesep 100 retains hydrophobic compounds by reversed-phase retention alone or a combination of reversed-phase and ion-exchange retention. Primesep 100 separates underivatized amino acids and amines by combining reversed-phase and ion-exchange mechanisms. In this case the acidic functional group acts as an embedded ion-pairing reagent. Organic acids are separated by reversed phase and ion exclusion. Inorganic cations can be separated by cation exchange. Methods can be developed on Primesep columns that are fully compatible with detectors such as UV, RI, conductivity, fluorescence, evaporative light scattering (ELSD) as well as mass spectrometers (LC/MS) and preparative chromatography (prep HPLC) systems.

Application Analytes:

Amino Sugar

Application Detection:

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