|
HPLC Application: Separation of Steroids in Reverse and Normal Mode on Primesep 100 Column
 |
|
Application Notes: The separation of steroids on Primesep 100 demonstrates the versatility of Primesep columns in both reversed- and normal-phase modes. Switching modes by changing the mobile phase reverses peaks 2 and 3. The separation method uses a mobile phase mixture of water and acetonitrile (MeCN, ACN) for reversed phase and hexane and ethanol for normal phase with UV detection at 240 nm. Application Analytes: Application Detection: |
SIELC Technologies | 65 East Palatine Road, Suite 221, Prospect Heights, IL USA 60070 | Tel: (847) 229-2629 Fax: (847) 655-6079 | Email: mail@sielc.com | sitemap
Copyright © SIELC Technologies. 2002-2007
Copyright © SIELC Technologies. 2002-2007