HPLC Application: Separation of Two Vitamins: Different Polarity - Isocratic Methods

Separation of Separation of Two Vitamins: Different Polarity - Isocratic Methods

Application Notes:

Primesep B and B2 columns separate Vitamins C (ascorbic acid) and B2 (riboflavin) with tunable selectivity. Peak order reversal is exhibited on these two columns with the same mobile phases due to their different polarity. The HPLC separation uses a mobile phase of water, acetonitrile (MeCN, ACN) and either formic or acetic acid (HOAc) with UV detection at 250 nm.

Application Analytes:

Vitamin B2 (Riboflavin)
Riboflavin (Vitamin B2)
Vitamin C
Ascorbic Acid

Application Detection:

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