HPLC Application: Effect of Buffer Concentration on Resolution of Neutral Compounds (Prednisolone and D-Glucose Pentaacetate)

Application Notes:

Application Columns:

Primesep^® P is a mixed-mode HPLC column with hydrophobic aromatic and ion-exchange properties. The Primesep P stationary phase combines a phenyl ring and an acidic functional group as a cation exchanger. The phenyl ring separates aromatic compounds by differences in pi-pi interaction. Primesep P retains basic compounds by a cation-exchange mechanism. Methods can be developed on Primesep columns that are fully compatible with detectors such as UV, RI, conductivity, fluorescence, evaporative light scattering (ELSD) as well as mass spectrometers (LC/MS) and preparative chromatography (prep HPLC) systems.

Application Analytes:

Application Detection:

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