| |
 |
Application Notes:
Application Columns:
- Primesep® B2 is a mixed-mode HPLC column with general hydrophobic and ion-exchange properties. The Primesep B2 stationary phase combines a hydrophobic chain and a basic functional group as an anion exchanger. Acids, bases, zwitterions and neutrals can be retained and separated by Primesep B2 columns. Primesep B2 also separates bases by an ion-exclusion mechanism. Primesep B2 demonstrates significant improvement in retention and selectivity for the separation of weak acid analytes by a combination of reversed phase and anion exchange. In this case the basic functional group acts as an embedded ion-pairing reagent. Primesep B2 column is one of the best separation media for natural product extracts. Methods can be developed on Primesep columns that are fully compatible with detectors such as UV, RI, conductivity, fluorescence, evaporative light scattering (ELSD) as well as mass spectrometers (LC/MS) and preparative chromatography (prep HPLC) systems.
- Primesep® C is a mixed-mode HPLC column with reversed-phase, ion-exchange and host-guest complex formation properties. The Primesep C stationary phase combines a hydrophobic chain and an acidic functional group as a cation exchanger. Acids, bases, and neutrals can be retained and separated by Primesep 100 columns. Primesep C retains hydrophobic compounds by a reversed phase. Strongly polar and basic compounds such as quaternary amines are retained without the use of ion-pairing reagent by reversed phase and ion exchange. An unusual elution order compared to other HPLC columns is found for primary, secondary, and tertiary amines. Methods can be developed on Primesep columns that are fully compatible with detectors such as UV, RI, conductivity, fluorescence, evaporative light scattering (ELSD) as well as mass spectrometers (LC/MS) and preparative chromatography (prep HPLC) systems.
- Primesep® D is a reversed-phase HPLC column with general hydrophobic and ion-exchange properties for direct injection of plasma samples. The Primesep D stationary phase combines a hydrophobic chain and a basic functional group as an anion exchanger. Primesep D excludes high molecular weight plasma compounds while retaining hydrophobic and acidic small molecules. Methods can be developed on Primesep columns that are fully compatible with detectors such as UV, RI, conductivity, fluorescence, evaporative light scattering (ELSD) as well as mass spectrometers (LC/MS) and preparative chromatography (prep HPLC) systems.
Application Analytes:
Application Detection:
|
|
|
