HPLC Application: HPLC Analysis of Polar Basic and Acetic Compounds on Primesep AB Column

Application Notes:

Application Columns:

Primesep^® AB is a mixed-mode HPLC column with general hydrophobic and ion-exchange properties. The Primesep AB stationary phase combines a hydrophobic chain and both acidic and basic functional groups as ion exchangers. Acids, bases, zwitterions and neutrals can be retained and separated by Primesep AB columns. Primesep AB separates bases by cation exchange and acids by anion exchange. Polar compounds such as acids and bases can be separated in one method. Methods can be developed on Primesep columns that are fully compatible with detectors such as UV, RI, conductivity, fluorescence, evaporative light scattering (ELSD) as well as mass spectrometers (LC/MS) and preparative chromatography (prep HPLC) systems.
Ordinary C18 Columns

Application Analytes:

Application Detection:

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