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Application Notes:
Application Columns:
- Primesep® A is a mixed-mode HPLC column with general hydrophobic and ion-exchange properties. The Primesep A stationary phase combines a hydrophobic chain and a strong acid functional group as a cation exchanger. Acids, bases, zwitterions and neutrals can be retained and separated by Primesep A columns. Primesep A retains hydrophobic compounds by reversed-phase retention alone or a combination of reversed-phase and ion-exchange retention. Primesep A separates underivatized amino acids, amines and weak bases by combining reversed-phase and ion-exchange mechanisms. In this case the acidic functional group acts as an embedded ion-pairing reagent. Organic acids are separated by reversed phase and ion exclusion. Inorganic cations can be separated by cation exchange. Methods can be developed on Primesep columns that are fully compatible with detectors such as UV, RI, conductivity, fluorescence, evaporative light scattering (ELSD) as well as mass spectrometers (LC/MS) and preparative chromatography (prep HPLC) systems.
- Primesep® D is a reversed-phase HPLC column with general hydrophobic and ion-exchange properties for direct injection of plasma samples. The Primesep D stationary phase combines a hydrophobic chain and a basic functional group as an anion exchanger. Primesep D excludes high molecular weight plasma compounds while retaining hydrophobic and acidic small molecules. Methods can be developed on Primesep columns that are fully compatible with detectors such as UV, RI, conductivity, fluorescence, evaporative light scattering (ELSD) as well as mass spectrometers (LC/MS) and preparative chromatography (prep HPLC) systems.
Application Analytes:
Application Detection:
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