HPLC Application: HPLC Separation of Glucosamine from Chondroitin Sulfate

Separation of HPLC Separation of Glucosamine from Chondroitin Sulfate

Application Notes:

Glucosamine is an amino sugar believed to play a role in cartilage formation and repair. Chondroitin sultate gives cartilage elasticity. Primesep 500 separates glucosamine and chondroitin sulfate with baseline resolution. Chondroitin sulfate is separated from chloride ion by ion exclusion and glucosamine is separated by sodium ion by ion exchange and reversed phase. The HPLC separation uses a mobile phase of water, acetonitrile (MeCN, ACN), and ammonium formate with evaporative light scattering detection (ELSD) with a column temperature of 90C.

Application Columns:

Primesep^® 500 is a mixed-mode HPLC column with general hydrophobic and ion-exchange properties. The Primesep 500 stationary phase combines a hydrophobic chain and a weak carboxylic acid functional group with a pKa of 5 as a cation exchanger. Acids, bases, and neutrals can be retained and separated by Primesep 500 columns. Primesep 500 retains hydrophobic compounds by reversed-phase retention alone or a combination of reversed-phase and ion-exchange retention. Primesep 500 retains basic compounds by reversed phase at mobile phase pH < 5 and separates acids by anion exclusion at mobile phase pH > 5. Methods can be developed on Primesep columns that are fully compatible with detectors such as UV, RI, conductivity, fluorescence, evaporative light scattering (ELSD) as well as mass spectrometers (LC/MS) and preparative chromatography (prep HPLC) systems.

Application Analytes:

Application Detection:

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