HPLC Application: HPLC Separation of Saccharin and Sorbitol

Application Notes:

Application Columns:

Primesep^® N is a normal-phase HPLC column with embedded acidic groups with a pKa of about 5. The Primesep N stationary phase retains basic compounds by cation exchange at pH > 5. Primesep N separates polar compounds by a HILIC (hydrophilic liquid chromatography) mechanism. Methods can be developed on Primesep columns that are fully compatible with detectors such as UV, RI, conductivity, fluorescence, evaporative light scattering (ELSD) as well as mass spectrometers (LC/MS) and preparative chromatography (prep HPLC) systems.

Application Analytes:

Application Detection:

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