HPLC Application: HILIC Retention of 1,5-naphalenedisulfonic Acid on Obelisc N Mixed-Mode HILIC Columns

Separation of HILIC Retention of 1,5-naphalenedisulfonic Acid on Obelisc N Mixed-Mode HILIC Columns

Application Notes:

Naphalenedisulfonic acid is retained by anion-exchange mechanism on Obelisc N mixed-mode HILIC column. Retention time is controlled by amount of ion in the mobile phase. Method demonstrates very good peak shape and controllable retention. This mixed-mode approach can be used for quantitation of various sulfonic acid in different matrices. UV, ELSD or LC/MS can be used for quantitation.

Application Analytes:

Sulfonic Acid
1,5-Naphalenedisulfonic Acid

Application Detection:

ELSD
LC/MS
UV

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