| CAS Number | 83905-01-5 |
|---|---|
| Molecular Formula | C38H72N2O12 |
| Molecular Weight | 748.996 |
| InChI Key | MQTOSJVFKKJCRP-BICOPXKESA-N |
| LogP | 4.02 |
| Synonyms |
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Applications:
Uv-Vis Spectrum of Azithromycin
March 5, 2026
Access the UV-Vis Spectrum SIELC Library
If you are looking for optimized HPLC method to analyze Azithromycin check our HPLC Applications library
For optimal results in HPLC analysis, it is recommended to measure absorbance at a wavelength that matches the absorption maximum of the compound(s) being analyzed. The UV spectrum shown can assist in selecting an appropriate wavelength for your analysis. Please note that certain mobile phases and buffers may block wavelengths below 230 nm, rendering absorbance measurement at these wavelengths ineffective. If detection below 230 nm is required, it is recommended to use acetonitrile and water as low UV-transparent mobile phases, with phosphoric acid and its salts, sulfuric acid, and TFA as buffers.
For some compounds, the UV-Vis Spectrum is affected by the pH of the mobile phase. The spectra presented here are measured with an acidic mobile phase that has a pH of 3 or lower.
HPLC Method for Determination of Azithromycin on Primesep 200 Column
September 2, 2022
HPLC Method for Azithromycin on Primesep 200 by SIELC Technologies
High Performance Liquid Chromatography (HPLC) Method for Analysis of Azithromycin.
Azithromycin is an antibiotic with the chemical formula C38H72N2O12. It is used in the treatment of various bacterial infections including but not limited to pneumonia, strep throat, and STI. As a medication, it is available in a variety of forms: tablets, capsules, injections, and solutions. It is on the WHOs list of essential medicines and is classified as critically important.
In reversed-phase HPLC, it generally exhibits significant tailing. The tailing can be substantially reduced by using a mixed-mode Primesep 200 column with a simple MS-compatible mobile phase of water, acetonitrile (ACN) and ammonium formate (AmFm) buffer.
| Column | Primesep 200, 2.1 x 50 mm, 5 µm, 100 A, dual ended |
| Mobile Phase | MeCN – 75% |
| Buffer | Ammonium Formate pH 3.0 – 50 mM |
| Flow Rate | 0.2 ml/min |
| Detection | ELSD, 50C |
| Class of Compounds | Drug |
| Analyzing Compounds | Azithromycin |
Application Column
Primesep 200
Column Diameter: 2.1 mm
Column Length: 50 mm
Particle Size: 5 µm
Pore Size: 100 A
Column options: dual ended
HPLC Determination of Azithromycin on Primesep SB Column
September 9, 2020
HPLC Method for Azithromycin on Primesep SB by SIELC Technologies
High Performance Liquid Chromatography (HPLC) Method for Analysis of Azithromycin.
Azithromycin is an antibiotic used in the treatment of various bacterial infections. It is on the WHOs list of essential medicines and is classified as critically important. In reversed-phase HPLC, it generally exhibits significant tailing. The tailing can be substantially reduced by using a mixed-mode Primesep SB column with a simple MS-compatible mobile phase of water, acetonitrile (ACN) and ammonium formate (AmFm) buffer.
| Column | Primesep SB, 4.6 x 150 mm, 5 µm, 100 A, dual ended |
| Mobile Phase | MeCN/H2O – 15/85% |
| Buffer | AmFm pH 3.0 – 50 mM |
| Flow Rate | 1.0 ml/min |
| Detection | CAD |
| Class of Compounds |
Drug, Antibiotics |
| Analyzing Compounds | Azithromycin |
Application Column
Primesep SB
Column Diameter: 4.6 mm
Column Length: 150 mm
Particle Size: 5 µm
Pore Size: 100 A
Column options: dual ended
Separation of Azithromycin on Newcrom R1 HPLC column
May 16, 2018
Azithromycin can be analyzed by this reverse phase (RP) HPLC method with simple conditions. The mobile phase contains an acetonitrile (MeCN), water, and phosphoric acid. For Mass-Spec (MS) compatible applications the phosphoric acid needs to be replaced with formic acid. Smaller 3 µm particles columns available for fast UPLC applications. This liquid chromatography method is scalable and can be used for isolation impurities in preparative separation. It also suitable for pharmacokinetics.
Application Column
Newcrom R1
The Newcrom columns are a family of reverse-phase-based columns. Newcrom A, AH, B, and BH are all mixed-mode columns with either positive or negative ion-pairing groups attached to either short (25 Å) or long (100 Å) ligand chains. Newcrom R1 is a special reverse-phase column with low silanol activity.
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