| CAS Number | 50-28-2 |
|---|---|
| Molecular Formula | C18H24O2 |
| Molecular Weight | 272.388 |
| InChI Key | VOXZDWNPVJITMN-ZBRFXRBCSA-N |
| LogP | 4.01 |
| Synonyms |
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Applications:
UV-Vis Spectrum of B-Estradiol
July 11, 2025
For optimal results in HPLC analysis, it is recommended to measure absorbance at a wavelength that matches the absorption maximum of the compound(s) being analyzed. The UV spectrum shown can assist in selecting an appropriate wavelength for your analysis. Please note that certain mobile phases and buffers may block wavelengths below 230 nm, rendering absorbance measurement at these wavelengths ineffective. If detection below 230 nm is required, it is recommended to use acetonitrile and water as low UV-transparent mobile phases, with phosphoric acid and its salts, sulfuric acid, and TFA as buffers.
For some compounds, the UV-Vis Spectrum is affected by the pH of the mobile phase. The spectra presented here are measured with an acidic mobile phase that has a pH of 3 or lower.
HPLC Method for Analysis of B-Estradiol on Primesep 200 Column
October 8, 2018
B-Estradiol is an estrogen steroid hormone. It is the major estrogen secreted by the premenopausal ovary. It is synthesized from testosterone primarily in the ovarian granulosa cells and placenta. B-estradiol is used as a medication, for instance in menopausal hormone therapy. Primesep 200, a reverse phase column, contains embedded acidic ionizable groups and can retain B-Estradiol. The method is UV compatible and can be used as a general approach for analyzing similar compounds.
| Column | Primesep 200, 3.2×100 mm, 5 µm, 100A |
| Mobile Phase | Gradient MeCN – 5-50%, 5 min, 4 min hold |
| Buffer | Gradient H2SO4 – 0.05-0.3 %, 5 min, 4 min hold |
| Flow Rate | 1.0 ml/min |
| Detection | UV, 270 nm |
| Class of Compounds |
Drug, Hormone, Hydrophobic |
| Analyzing Compounds | B-Estradiol |
Application Column
Primesep 200
The Primesep family of mixed-mode columns offers a wide variety of stationary phases, boasting unprecedented selectivity in the separation of a broad array of chemical compounds across multiple applications. Corresponding Primesep guard columns, available with all stationary phases, do not require holders. SIELC provides a method development service available to all customers. Inquire about our specially-tailored custom LC-phases for specific separations.
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HPLC Method for Analysis of B-Estadiol
April 29, 2016
| Column | Primesep 200, 3.2×100 mm, 5 µm, 100A |
| Mobile Phase | Gradient MeCN – 5-50% |
| Buffer | Gradient H3PO4 – 0.05-0.3%, 5 min, 4 min hold |
| Flow Rate | 0.5 ml/min |
| Detection | UV, 270 nm |
| Class of Compounds |
Drug, Acid, Hydrophilic, Ionizable, |
| Analyzing Compounds | B-Estadiol |
Application Column
Primesep 200
The Primesep family of mixed-mode columns offers a wide variety of stationary phases, boasting unprecedented selectivity in the separation of a broad array of chemical compounds across multiple applications. Corresponding Primesep guard columns, available with all stationary phases, do not require holders. SIELC provides a method development service available to all customers. Inquire about our specially-tailored custom LC-phases for specific separations.
Select options
