Azinphos-ethyl

Azinphos-ethyl structural formula

CAS Number2642-71-9
Molecular FormulaC12H16N3O3PS2
Molecular Weight345.370
InChI KeyRQVGAIADHNPSME-UHFFFAOYSA-N
LogP3.40
Synonyms
  • Azinphos-ethyl
  • O,O-Diethyl S-[(4-oxo-1,2,3-benzotriazin-3(4H)-yl)methyl] phosphorodithioate
  • Phosphorodithioic acid, O,O-diethyl S-[(4-oxo-1,2,3-benzotriazin-3(4H)-yl)methyl] ester
  • 2642-71-9
  • Athyl-gusathion
  • Azinophos-ethyl
  • Azinugec E
  • Bayer 16259
  • Benzotriazine derivative of an ethyl dithiophosphate
  • BRN 0297468
  • Caswell No. 344
  • Cotnion-ethyl
  • Crysthion
  • 3-Diethoxyphosphinothioylthiomethyl-1,2,3-benzotriazin-4(3H)-one
  • O,O-Diethyl S-(4-oxobenzotriazino-3-methyl)phosphorodithioate
  • O,O-Diethyl S-(4-oxobenzyltriazine-3-methyl) dithiophosphate
  • O,O-Diethyl phosphorodithioate S-ester with 3-(mercaptomethyl)-1,2,3-benzotriazin-4(3H)-one
  • O,O-Diethyl-S-(4-oxo-3H-1,2,3-benzotriazine-3-yl)-methyl-dithiophosphate
  • 3-Diethyloxyphosphinothioylthiomethyl-1,2,3-benzotriazin-4(3H)-one
  • S-3,4-Dihydro-4-oxobenzo(d)(1.2.3)triazin-3-ylmethyl O,O-diethyl phosphorodithioate
  • S-(3,4-Dihydro-4-oxo-1,2,3-benzotriazin-3-ylmethyl) O,O-diethyl phosphorodithioate
  • 3,4-Dihydro-4-oxo-3-benzotriazinylmethyl O,O-diethyl phosphorodithioate
  • EINECS 220-147-6
  • EPA Pesticide Chemical Code 058002
  • Ethyl Gusathion
  • Ethyl guthion
  • Ethyl homolog of guthion
  • Gusathion A
  • Gusathion A-M
  • Gusathion H
  • Gusathion H and K
  • Gusathion K forte
  • Gusation A
  • Sepizin L
  • Azinfos-ethyl
  • Azinphos-aethyl
  • Azinphos-etile
  • O,O-Diaethyl-S-((4-oxo-3H-1,2,3-benzotriazin-3-yl)-methyl)-dithiophosphat
  • O,O-Diaethyl-S-(4-oxobenzotriazin-3-methyl)-dithiophosphat
  • O,O-Diethyl-S-((4-oxo-3H-1,2,3-benzotriazin-3-yl)-methyl)-dithio fosfaat
  • O,O-Diethyl-S-((4-oxo-3H-1,2,3-benzotriazin-3-yl)-methyl)-dithiofosfaat
  • O,O-Dietil-S-((4-oxo-3H-1,2,3-benzotriazin-3-il)-metil)-ditiofosfato
  • Triazotion
  • UNII-EA96NYT5J5
  • Azinphos ethyl
  • Ethyl azinphos
  • Gusathion
  • O,O-Diethyl S-((4-oxo-1,2,3-benzotriazin-3(4H)-yl)methyl) phosphorodithioate
  • Phosphorodithioic acid, O,O-diethyl S-((4-oxo-1,2,3-benzotriazin-3(4H)-yl)methyl) ester

Applications:

Controlling Retention of Azinphos-ethyl on Primesep and Obelisc HPLC Columns

September 14, 2015

 

Azinphos-ethyl is a toxic pesticide in the organophosphate class. It is currently on the WHO (World Health Organization) hazard classification as class IB, highly hazardous. Azinphos-methyl, a compound with two less carbons is also a pesticide and considered a target pesticide by the EURL (European Union Reference Laboratory) for the EUPT-FF9 2015 a proficiency test for the analysis of a over a hundred pesticides. This compound was separated on two different mixed-mode columns, Primesep 100 and Obelisc R. Primesep 100 is a reverse phase column that contains embedded acidic ion-pairing groups, while Obelisc R retains with long hydrophobic chains and multiple ionic pairing groups on the surface. Method is LC/MS compatible and can be replicated to analyze dozens of other pesticides.

Condition 1

Column Obelisc R, 2.1×150 mm, 5 µm, 100A
Mobile Phase Gradient MeCN – 25-70%, 15 min
Buffer Gradient AmAc pH 3.0- 30-60 mM, 15 min
Flow Rate 0.4 ml/min
Detection UV, 270 nm

 

Condition 2

Column Obelisc R, 2.1×150 mm, 5 µm, 100A
Mobile Phase Gradient MeCN – 10-70%, 15 min
Buffer Gradient AmAc pH 3.0- 20-60 mM, 15 min
Flow Rate 0.4 ml/min
Detection UV, 270 nm

 

Condition 3

Column Primesep 100, 2.1×150 mm, 5 µm, 100A
Mobile Phase Gradient MeCN – 10-70%, 15 min
Buffer Gradient AmAc pH 3.0- 20-60 mM, 15 min
Flow Rate 0.4 ml/min
Detection UV, 270 nm

 

Description

Class of Compounds
 Insecticide, Herbicide, Fungicide,  Hydrophobic, Ionizable
Analyzing Compounds Azinphos-ethyl

 

Application Column

Obelisc R

SIELC has developed the Obelisc™ columns, which are mixed-mode and utilize Liquid Separation Cell technology (LiSC™). These cost-effective columns are the first of their kind to be commercially available and can replace multiple HPLC columns, including reversed-phase (RP), AQ-type reversed-phase, polar-embedded group RP columns, normal-phase, cation-exchange, anion-exchange, ion-exclusion, and HILIC (Hydrophilic Interaction Liquid Chromatography) columns. By controlling just three orthogonal method parameters - buffer concentration, buffer pH, and organic modifier concentration - users can adjust the column properties with pinpoint precision to separate complex mixtures.

Select options

Primesep 100

The Primesep family of mixed-mode columns offers a wide variety of stationary phases, boasting unprecedented selectivity in the separation of a broad array of chemical compounds across multiple applications. Corresponding Primesep guard columns, available with all stationary phases, do not require holders. SIELC provides a method development service available to all customers. Inquire about our specially-tailored custom LC-phases for specific separations.

Select options
Application Analytes:
Azinphos-ethyl

Application Detection:
UV Detection
SIELC Technologies usually develops more than one method for each compound. Therefore, this particular method may not be the best available method from our portfolio for your specific application. Before you decide to implement this method in your research, please send us an email to research@sielc.com so we can ensure you get optimal results for your compound/s of interest.

Azinphos-ethyl Analysis on Mixed-Mode HPLC Columns

August 24, 2015

Azinphos-ethyl is a highly toxic organophosphate insecticide.  Azinphos-ethyl was retained on both Obelisc R and Primesep B2 mixed-mode HPLC columns. Primesep B2 contains embedded basic ion-pairing groups while Obelisc R contains embedded ionic and hydrophobic groups which can assist in fine tuning separations. Method is LC/MS compatible and can be used as a general approach for analyzing azinphos-ethyl and other organophosphate insecticides.

Condition 1

Column Primesep B2, 2.1×150 mm, 5 µm, 100A
Mobile Phase Gradient MeCN – 25-70%, 15 min,  7 min hold
Buffer Gradient AmAc pH 3.0- 30-60 mM, 15 min,  7 min hold
Flow Rate 0.4 ml/min
Detection UV, 250 nm

 

Condition 2

Column Obelisc R, 2.1×150 mm, 5 µm, 100A
Mobile Phase Gradient MeCN – 10-70%, 15 min,  7 min hold
Buffer Gradient AmAc pH 3.0- 20-60 mM, 15 min,  7 min hold
Flow Rate 0.4 ml/min
Detection UV, 250 nm

 

 

Description

Class of Compounds
 Insecticide, Herbicide, Fungicide,  Hydrophobic, Ionizable
Analyzing Compounds Azinphos-ethy

 

Application Column

Obelisc R

SIELC has developed the Obelisc™ columns, which are mixed-mode and utilize Liquid Separation Cell technology (LiSC™). These cost-effective columns are the first of their kind to be commercially available and can replace multiple HPLC columns, including reversed-phase (RP), AQ-type reversed-phase, polar-embedded group RP columns, normal-phase, cation-exchange, anion-exchange, ion-exclusion, and HILIC (Hydrophilic Interaction Liquid Chromatography) columns. By controlling just three orthogonal method parameters - buffer concentration, buffer pH, and organic modifier concentration - users can adjust the column properties with pinpoint precision to separate complex mixtures.

Select options

Primesep B2

The Primesep family of mixed-mode columns offers a wide variety of stationary phases, boasting unprecedented selectivity in the separation of a broad array of chemical compounds across multiple applications. Corresponding Primesep guard columns, available with all stationary phases, do not require holders. SIELC provides a method development service available to all customers. Inquire about our specially-tailored custom LC-phases for specific separations.

Select options
Application Analytes:
Azinphos-ethyl

Application Detection:
UV Detection
SIELC Technologies usually develops more than one method for each compound. Therefore, this particular method may not be the best available method from our portfolio for your specific application. Before you decide to implement this method in your research, please send us an email to research@sielc.com so we can ensure you get optimal results for your compound/s of interest.

HPLC Analysis of Azinphos-ethyl

August 10, 2015

Azinphos-ethyl is an organophosphate insecticide similar to Azinphos-methyl which was developed during World War II as a nerve agent. Due to risk concerns for farm workers and aquatic ecosystems AZM use was phased-out by the EPA in 2012. AZM is on the EURL–SRM list of target pesticides in cereals and feeding stuffs. Azinphos-ethyl was retained on Obelisc R, a mixed-mode HPLC column which contains ionic groups and hydrophobic chain. Method is LC/MS compatible and can be used as a general approach for analyzing azinphos-ethyl and other organophosphate insecticides.

Condition

Column Obelisc R, 4.6×150 mm, 5 µm, 100A
Mobile Phase MeCN – 40%
Buffer AmFm pH 3.0- 20 mM
Flow Rate 1.0 ml/min
Detection UV, 250 nm

 

 

Description

Class of Compounds
 Insecticide, Herbicide, Fungicide,  Hydrophobic, Ionizable
Analyzing Compounds Azinphos-ethyl

 

Application Column

Obelisc R

SIELC has developed the Obelisc™ columns, which are mixed-mode and utilize Liquid Separation Cell technology (LiSC™). These cost-effective columns are the first of their kind to be commercially available and can replace multiple HPLC columns, including reversed-phase (RP), AQ-type reversed-phase, polar-embedded group RP columns, normal-phase, cation-exchange, anion-exchange, ion-exclusion, and HILIC (Hydrophilic Interaction Liquid Chromatography) columns. By controlling just three orthogonal method parameters - buffer concentration, buffer pH, and organic modifier concentration - users can adjust the column properties with pinpoint precision to separate complex mixtures.

Select options
Application Analytes:
Azinphos-ethyl

Application Detection:
UV Detection
SIELC Technologies usually develops more than one method for each compound. Therefore, this particular method may not be the best available method from our portfolio for your specific application. Before you decide to implement this method in your research, please send us an email to research@sielc.com so we can ensure you get optimal results for your compound/s of interest.