If you are looking for optimized HPLC method to analyze Benzenesulfonamide check our HPLC Applications library

For optimal results in HPLC analysis, it is recommended to measure absorbance at a wavelength that matches the absorption maximum of the compound(s) being analyzed. The UV spectrum shown can assist in selecting an appropriate wavelength for your analysis. Please note that certain mobile phases and buffers may block wavelengths below 230 nm, rendering absorbance measurement at these wavelengths ineffective. If detection below 230 nm is required, it is recommended to use acetonitrile and water as low UV-transparent mobile phases, with phosphoric acid and its salts, sulfuric acid, and TFA as buffers.
For some compounds, the UV-Vis Spectrum is affected by the pH of the mobile phase. The spectra presented here are measured with an acidic mobile phase that has a pH of 3 or lower.

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Both Benzenesulfonamide and sulfanilamide are organic antibacterial compounds differing only in the addition of an extra amino group in sulfanilamide. The closely related compounds can be separated on a mixed-mode Primesep D column by hydrophobic and anion-exchange mechanisms or Primesep 100 column by hydrophobic and cation-exchange mechanisms with the elution order reversed using identical mobile phases. UV Detection at 230nm.

Primesep 100 separates a mixture of sulfonamides (benzenesulfonamide, chlorobenzenesulfonamide, hydroxybenzenesulfonamide) and hydroxysulphaminophenylhydrazine by a mixture of polar and hydrophobic interactions. The stationary phase’s hydrophobic functionality provides a reversed-phase mechanism while the embedded cation-exchange group provides polar interactions. The separation method uses a mobile phase mixture of water, acetonitrile (MeCN, ACN) and trifluoracetic acid (TFA) with UV detection at 210 nm. This method is mass spec (LC/MS) and evaporative light scattering (ELSD) compatible.