beta-Aminobutyric Acid

beta-Aminobutyric Acid structural formula

CAS Number541-48-0
Molecular FormulaC4H9NO2
Molecular Weight103.122
InChI KeyOQEBBZSWEGYTPG-UHFFFAOYSA-N
LogP-1.79
Synonyms
  • beta-Aminobutyric acid
  • 3-Aminobutanoic acid
  • Butanoic acid, 3-amino-
  • 541-48-0
  • BABA
  • EINECS 208-783-2
  • EINECS 220-617-0
  • UNII-4282SA5CTS
  • 3-Aminobutyric acid
  • 3-aminobutanoic acid
  • 3-methyl-beta-alanine
  • 167222-96-0
  • 2835-82-7

Applications:

HPLC Separation of alpha-Aminobutyric, beta-Aminobutyric, and gamma-Aminobutyric acids on Obelisc N

March 3, 2010


GABA (neurotransmitter) and its isomers are polar zwitter-ionic compounds. Due to the position of amino-groups, all three compounds show different polar and basic properties. The isomers of aminobuturic acid are separated on an Obelisc N HILIC/cation-exchange column. Buffer concentration has a different effect on retention of alpha-, beta-, and gamma-aminobutyric acid. This general and robust method can be used for separation of other polar and ionizable compounds and isomers by mixed-mode chromatography.

Condition

Column Obelisc N,  4.6×150 mm, 5 µm, 100A
Mobile Phase MeCN/H2O
Buffer AmFm pH 3.0
Flow Rate 1.0 ml/min
Detection ELSD

 

Description

Class of Compounds
Acid
Analyzing Compounds Alpha-Aminobutyric acid, Beta-Aminobutryic acid, Gamma-Aminobutyric acid (GABA)

Application Column

Obelisc N

SIELC has developed the Obelisc™ columns, which are mixed-mode and utilize Liquid Separation Cell technology (LiSC™). These cost-effective columns are the first of their kind to be commercially available and can replace multiple HPLC columns, including reversed-phase (RP), AQ-type reversed-phase, polar-embedded group RP columns, normal-phase, cation-exchange, anion-exchange, ion-exclusion, and HILIC (Hydrophilic Interaction Liquid Chromatography) columns. By controlling just three orthogonal method parameters - buffer concentration, buffer pH, and organic modifier concentration - users can adjust the column properties with pinpoint precision to separate complex mixtures.

Select options
Application Analytes:
Zwitterion
alpha-Aminobutyric Acid
beta-Aminobutyric Acid
gamma-Aminobutyric Acid (GABA)

Application Detection:
ELSD Detection
SIELC Technologies usually develops more than one method for each compound. Therefore, this particular method may not be the best available method from our portfolio for your specific application. Before you decide to implement this method in your research, please send us an email to research@sielc.com so we can ensure you get optimal results for your compound/s of interest.

HPLC Separation of Isomers of Aminobutyric Acids

May 6, 2004

Primesep C separates the isomers of aminobutyric acids by a combination of reversed-phase and ionic interaction mechanisms. alpha-Aminobutyric acid, beta-Aminobutryic acid, and gamma-Aminobutyric acid (GABA) are baseline resolved without ion-pair reagents. The HPLC separation uses a mobile phase of water, acetonitrile (MeCN, ACN) ammonium acetate with evaporative light scattering detection (ELSD).

Condition

Column Primesep C,  3.2×150 mm, 5 µm, 100A
Mobile Phase MeCN/H2O
Buffer AmAc pH 4.1
Flow Rate 0.5 ml/min
Detection ELSD

 

Description

Class of Compounds
Acid
Analyzing Compounds Alpha-Aminobutyric acid, Beta-Aminobutryic acid, Gamma-Aminobutyric acid (GABA)

Application Column

Primesep C

The Primesep family of mixed-mode columns offers a wide variety of stationary phases, boasting unprecedented selectivity in the separation of a broad array of chemical compounds across multiple applications. Corresponding Primesep guard columns, available with all stationary phases, do not require holders. SIELC provides a method development service available to all customers. Inquire about our specially-tailored custom LC-phases for specific separations.

Select options
Application Analytes:
Aminobutyric Acids
alpha-Aminobutyric Acid
beta-Aminobutyric Acid
gamma-Aminobutyric Acid (GABA)

Application Detection:
ELSD Detection
SIELC Technologies usually develops more than one method for each compound. Therefore, this particular method may not be the best available method from our portfolio for your specific application. Before you decide to implement this method in your research, please send us an email to research@sielc.com so we can ensure you get optimal results for your compound/s of interest.