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For optimal results in HPLC analysis, it is recommended to measure absorbance at a wavelength that matches the absorption maximum of the compound(s) being analyzed. The UV spectrum shown can assist in selecting an appropriate wavelength for your analysis. Please note that certain mobile phases and buffers may block wavelengths below 230 nm, rendering absorbance measurement at these wavelengths ineffective. If detection below 230 nm is required, it is recommended to use acetonitrile and water as low UV-transparent mobile phases, with phosphoric acid and its salts, sulfuric acid, and TFA as buffers.
For some compounds, the UV-Vis Spectrum is affected by the pH of the mobile phase. The spectra presented here are measured with an acidic mobile phase that has a pH of 3 or lower.

Five antibiotics are separated on Primesep C mixed-mode cation-exchange column using UV, ESLD and LC/MS compatible conditions. Similar compounds can be separated based on reverse phase and cation-exchange mechanisms. Current method can be used in quantitative determination of antibiotics in various food products.

Fish antibiotics are used in fish farming to treat bacterial diseases of fish. It is common practice in the fish industry, particularly in developing countries, to use large amounts of antibiotics to prevent infection. The antibiotics used are often non-biodegradable and remain in the environment for long periods of time, contaminating soil and ground waters. In farming antibiotics are mixed with food and residual amount of drugs ends up in fish products and poultry, this lead to consumption of antibiotics and metabolites by humans. Five common antibiotics are separated on Primesep C column using LC/MS compatible conditions. Method can be used for quantitative determination of nitrofurantoin, nitrofurazone, furazolidone, furaltadone, chloramphenicol and nitrofurazone in fish products and environment.