gamma-Aminobutyric Acid (GABA)

gamma-Aminobutyric Acid (GABA) structural formula

CAS Number 56-12-2
Molecular Formula C4H9NO2
Molecular Weight 103.122 g/mol
InChI Key BTCSSZJGUNDROE-UHFFFAOYSA-N
LogP -3.17
Synonyms
  • 4-Aminobutanoic acid
  • Butanoic acid, 4-amino-
  • 56-12-2
  • Butanoic acid, 4-amino-
  • 3-Carboxypropylamine
  • 4-Aminobuttersaure
  • 4-aminobutyric acid
  • Acide 4-aminobutyrique
  • acido 4-aminobutirico
  • Aminalon
  • Butyric acid, 4-amino-
  • Gaballon
  • Gamarex
  • Gammalon
  • Gammalone
  • Gammasol
  • Mielogen
  • Mielomade
  • NSC 27418
  • NSC 32044
  • NSC 45460
  • NSC 51295
  • Pharmagaba
  • Pharmagaba 20
  • Pharmagaba 20D
  • Pharma-GABA 20S
  • Piperidic acid
  • Piperidinic acid
  • γ-Aminobutanoic acid
  • γ-Aminobutryic acid
  • γ-Aminobutyric acid
  • ω-Aminobutyric acid
  • EINECS 200-258-6
  • Gamastan
  • Gammagee
  • Gamulin
  • EPA Pesticide Chemical Code 030802
  • Acide amino-4- butyrique
  • UNII-2ACZ6IPC6I
  • Aminalone
  • 4-Aminobutanoate
  • 4Abu
  • GABA
  • Gammar
  • Omega-Aminobutyrate
  • Piperidate
  • Piperidinate
  • gamma Aminobutyrate
  • gamma Aminobutyric acid
  • gamma-Aminobuttersaeure
  • gamma-Aminobutyrate
  • gamma-amino-n-butyric acid
  • gamma-aminobutanoic acid
  • omega-aminobutyric acid
  • w-Aminobutyrate
  • w-Aminobutyric acid
  • gamma-aminobutyric acid
  • 4-ammoniobutanoate
  • gamma-aminobutyric acid zwitterion
  • 3131-86-0

Applications:


HPLC Separation of Isomers of Aminobutyric Acids



Primesep C separates the isomers of aminobutyric acids by a combination of reversed-phase and ionic interaction mechanisms. alpha-Aminobutyric acid, beta-Aminobutryic acid, and gamma-Aminobutyric acid (GABA) are baseline resolved without ion-pair reagents. The HPLC separation uses a mobile phase of water, acetonitrile (MeCN, ACN) ammonium acetate with evaporative light scattering detection (ELSD).



Application Analytes:

Aminobutyric Acids
alpha-Aminobutyric Acid
beta-Aminobutyric Acid
gamma-Aminobutyric Acid (GABA)

HPLC Separation of Polar Compounds





Application Analytes:


Aspartic Acid
Dopamine
Glutamic Acid
Norepinephrine
gamma-Aminobutyric Acid (GABA)

HPLC Separation of Amino Acids



Amino acids are building blocks for peptides and proteins. Serine is not essential to human diet and it is synthesized in human body. Methionine is not synthesized in human body and needs to be ingested. GABA is used to enhance growth of specified plants, prevent development of powdery mildew on grapes, and suppress certain other plant diseases. In humans GABA helps to maintain normal brain function. Serine, methylserine, GABA and methionine are separated on Primesep 100 column by mixed-mode mechanism. Amino acids are retained by combination of reverse phase and cation-exchange mechanisms. At lower pH carboxylic acid fragment of amino acid is suppressed and not ionized, making amino acids more basic and slightly more hydrophobic. Amount of acetonitrile, buffer concentration and buffer pH can be used to adjust retention time. Underivatized amino acids are well retained and separated with perfect peak shape and symmetry. Fast method can be used for UV, ESLD and LC/MS quantitation of serine, methylserine, GABA and methionine.



Application Analytes:

Methionine
Serine
a-Methylserine
gamma-Aminobutyric Acid (GABA)

HPLC Separation of Serine, a-Methylserine, GABA, and Methionine Mixture


Four underivatized amino acids (serine, methylserine, GABA and methionine) were separated on a Primesep 100 reversed-phase cation-exchange mixed-mode HPLC column. Two methods, one for UV and one for ELSD/LC/MS show good separation and peak shape for underivatized amino acids. This column and general HPLC approach can be used for analysis of underivatized amino acids. Primesep 100 is designed to replace reversed-phase HPLC column in combination with ion-pairing reagents.



Application Analytes:

Methionine
Serine
a-Methylserine
gamma-Aminobutyric Acid (GABA)

HPLC Separation of alpha-Aminobutyric, beta-Aminobutyric, and gamma-Aminobutyric acids on Obelisc N


GABA (neurotransmitter) and its isomers are polar zwitter-ionic compounds. Due to the position of amino-groups, all three compounds show different polar and basic properties. The isomers of aminobuturic acid are separated on an Obelisc N HILIC/cation-exchange column. Buffer concentration has a different effect on retention of alpha-, beta-, and gamma-aminobutyric acid. This general and robust method can be used for separation of other polar and ionizable compounds and isomers by mixed-mode chromatography.



Application Analytes:

Zwitterion
alpha-Aminobutyric Acid
beta-Aminobutyric Acid
gamma-Aminobutyric Acid (GABA)

HPLC Separation of GABA and GLU



Amino acids are building blocks for peptides and proteins; they are used in various supplement formulations and as starting reagents in chemistry to introduce chirality. GABA and GLU are used to enhance growth of specified plants, prevent development of powdery mildew on grapes, and suppress certain other plant diseases. L-Glutamic acid is one of the major amino acids naturally found in plant and animal proteins, and GABA helps to maintain normal brain function. Both amino acids behave as neurotransmitters. All amino acids have both basic and acidic groups. Depending on pH amino acids can be basic, acidic or zwitter-ionic. Due to the polar nature of underivatized amino acids, analysis is a very challenging task. Derivatization and ion-pairing reagents are used to provide retention of amino acids. Simple method is developed on Primesep 100 column using combination of acetonitrile/water with phosphoric acid as a mobile phase. Method uses UV detection. Amino acids are well retained without use of ion-pairing reagents. Fast reliable method can be developed for all underivatized natural and synthetic amino acids.



Application Analytes:

GLU (L-Glutamic acid)
gamma-Aminobutyric Acid (GABA)

HPLC Separation of Amino Acids in Supplements Composition in Mixed-Mode



Amino acids are essential components of numerous formulation. Health supplements can contain various amino acids and vitamins and require quantitation of each ingredients. Amino acids are very polar compounds with limited or no retention in reversed-phase chromatography. The most common approaches are reversed-phase chromatography with ion-pairing reagent and hydrophilic interaction chromatography (HILIC). Underivatized amino acids can be retained by combination of reversed-phase and cation exchange mechanism on Primesep 100 mixed-mode. Retention time is controlled by amount of acetonitrile, buffer and buffer pH. Method does not require ion-pairing reagent. This method is for UV detection. LC/MS, ELSD or Corona CAD can be employed for analysis of amino acids with trifluoroacteic acid or ammonium formate in the mobile phase. This approach can be used for HPLC analysis of all underivatized amino acids.



Application Analytes:


Amino Acids
Melatonin
Theanine
gamma-Aminobutyric Acid (GABA)

HPLC Separation of Glutamic Acid and GABA

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Glutamic acid and GABA are neurotransmitters. Glutamic acid and GABA are non-essential amino acids. They are hydrophilic and zwitter-ionic in nature . At lower pH, carboxylic acid groups of amino acids are not ionized, making them more hydrophobic and basic. Underivatized glutamic acid and GABA were retained and separated on a Primesep 100 column using ACN/water/TFA mobile phase. Amino acids can be monitored by low UV or ELSD/CAD. Retention is provided by reversed-phase and cation-exchange mechanism. Method can be used for analysis of underivatized amino acids in various matrices including supplements, vitamin and other complex mixtures. various mobile phase can be used with corresponding detection techniques.



Application Analytes:

Glutamic Acid
Zwitterion
gamma-Aminobutyric Acid (GABA)