| CAS Number | 51-45-6 |
|---|---|
| Molecular Formula | C5H9N3 |
| Molecular Weight | 111.149 |
| InChI Key | NTYJJOPFIAHURM-UHFFFAOYSA-N |
| LogP | -0.700 |
| Synonyms |
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Applications:
HPLC Method for Determination of Histamine on BIST A+ Column by SIELC Technologies
October 18, 2022
HPLC Method for Determination of Histamine on BIST A+ Column by SIELC Technologies
Separation type: Liquid Chromatography Mixed-mode
High Performance Liquid Chromatography (HPLC) Method for Analyses is of Histamine BIST A+ Column by SIELC Technologies
Histamine dihydrochloride, also known as Ceplene, is used to prevent a relapse of acute myeloid leukemia. Other pharmaceutical applications include as a topical analgesic to treat arthritis, aches, bruises, sprains, and strains. Brand names it is sold under include Australian Dream and Alo. Histamine dihydrochloride can be detected in the low UV regime. Using a BIST A+ column and a mobile phase consisting of water and Acetonitrile (MeCN) with an ammonium formate (AmFm) buffer, Histamine chloride can be retained, separated, and analyzed. This analysis method can be detected and is compatible with Mass Spectrometry (MS), ELSD, and CAD.
Condition
| Column | BIST A+, 4.6×150 mm, 5µm, 100A |
| Mobile Phase | MeCN – 70% |
| Buffer | Ammonium Formate pH 3.0 – 20 mM |
| Flow Rate | 1.0 ml/min |
| Detection | ELSD, 50C |
Description
| Class of Compounds | Drug |
| Analyzing Compounds | Histamine |
Application Column
BIST A+
BIST™ columns offer a unique and effective way to achieve separations that were traditionally challenging or even impossible with other HPLC columns. With the use of a special mobile phase, these ion exchange columns provide very strong retention for analytes with the same charge polarity as the stationary phase, unlocking new chromatography applications. What makes BIST™ columns stand out is their proprietary surface chemistry, which results in superior selectivity, resolution, and sensitivity. These columns offer a simple, efficient solution for a variety of analytical challenges, making them an excellent choice for researchers and analysts across many different fields. To learn more about the technology that powers BIST™ columns and to explore related applications, check out https://BIST.LC.
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HPLC Separation of Histamine and Phenethylamine
August 7, 2010
Histamine is a basic compound that acts like neurotransmitter. Phenethylamine is a natural amine alkaloid psychoactive compound with stimulant effects. It forms by enzymatic decarboxylation of phenylalanine, and also acts as neurotransmitter. Both compounds are separated in one HPLC run on Primesep C and Primesep 200 columns by reversed-phase cation-exchange mechanism. Primesep C provides shorter retention time and histamine and phenethylamine can be separated within 5 minutes. Primesep C and Primesep 200 columns do not require ion-pairing reagent as it is attached to the surface of Primesep silica gel. Method can be used for analysis of these two compounds in plasma, blood and urine with UV, ELSD, CAD or LC/MS detection.
Application Column
Primesep 200
The Primesep family of mixed-mode columns offers a wide variety of stationary phases, boasting unprecedented selectivity in the separation of a broad array of chemical compounds across multiple applications. Corresponding Primesep guard columns, available with all stationary phases, do not require holders. SIELC provides a method development service available to all customers. Inquire about our specially-tailored custom LC-phases for specific separations.
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Primesep C
The Primesep family of mixed-mode columns offers a wide variety of stationary phases, boasting unprecedented selectivity in the separation of a broad array of chemical compounds across multiple applications. Corresponding Primesep guard columns, available with all stationary phases, do not require holders. SIELC provides a method development service available to all customers. Inquire about our specially-tailored custom LC-phases for specific separations.
Select optionsPhenethylamine
