Homovanillic Acid

Homovanillic Acid structural formula

CAS Number 306-08-1
Molecular Formula C9H10O4
Molecular Weight 182.176 g/mol
InChI Key QRMZSPFSDQBLIX-UHFFFAOYSA-N
LogP 0.33
Synonyms
  • Homovanillic acid
  • (4-Hydroxy-3-methoxyphenyl)acetic acid
  • Benzeneacetic acid, 4-hydroxy-3-methoxy-
  • 306-08-1
  • Benzeneacetic acid, 4-hydroxy-3-methoxy-
  • (4-Hydroxy-3-methoxyphenyl)acetic acid
  • 3-Methoxy-4-hydroxyphenylacetic acid
  • 4-Hydroxy-3-methoxybenzeneacetic acid
  • 4-hydroxy-3-methoxyphenylacetic acid
  • 4-Hydroxy-3-methoxyphenylessigsaure
  • Acetic acid, (4-hydroxy-3-methoxyphenyl)-
  • Acide 4-hydroxy-3-methoxyphenylacetique
  • acido 4-hidroxi-3-metoxifenilacetico
  • NSC 16682
  • Vanilacetic acid
  • EINECS 206-176-7
  • UNII-X77S6GMS36
  • (4-Hydroxy-3-methoxyphenyl)acetate
  • 2-(4-hydroxy-3-methoxy-phenyl)acetic acid
  • 2-(4-hydroxy-3-methoxy-phenyl)ethanoic acid
  • 3-Methoxy-4-hydroxy-phenylacetic acid
  • 3-Methoxy-4-hydroxyphenylacetate
  • 3-Methoxy-4-hydroxyphenylacetic acid
  • 4-Hydroxy-3-methoxybenzeneacetate
  • 4-hydroxy 3-methoxyphenylacetic acid
  • HVA
  • Homovanilate
  • Homovanilic acid
  • Homovanillate
  • Homovanillinic acid
  • Vanilacetate
  • Vanillacetic acid

Applications:


HPLC Separation of Vanilmandelic, Hydroxyindoleacetic, and Homovanillic Acids





Application Analytes:

5-Hydroxyindole-3-Acetic Acid
Homovanillic Acid
Vanilmandelic Acid

Separation of Serotonin, Dopamine, and Related Compounds
Catecholamines are chemical compounds derived from the amino acid tyrosine containing catechol and amine groups. Some of them are biogenic amines. Retention of compounds of the catecholamine pathway is achieved on Obelisc N column. All polar compounds are well retained by combination of HILIC and ion-exchange mechanisms. Obelisc N columns produce very good peak shapes for all analytes. The method is very sensitive to amount of ACN, buffer and buffer pH. The retention time changes with variation of the main parameters. This method can be used for quantitation of biogenic amines and related compounds (homovanillic acid, dihydroxyphenyl acetic acid, serotonin, dopamine, epinephrine, hydroxytryptophan, epinephrine and DOPA) in urine, blood and other biological fluids. Further optimization of this HPLC method can be used during screening and validation. Amines and acids can be analyzed in the same run and retained by a combination of polar organic mode, cation-exchange and anion-exchange modes. Various buffers within specified pH can be employed (ammonium formate, ammonium acetate, sodium phosphate, etc.).

Application Analytes:

DOPA (3,4-dihydroxy-L-phenylalanine)
DOPAC (Dihydroxyphenylacetic Acid)
Dopamine
Epinephrine
Homovanillic Acid
Hydroxytryptophan
Norepinephrine
Serotonin

HPLC Separation of Homovanillic Acid, Dopamine, and DOPAC using Hydrogen Bonding



Application Analytes:

DOPAC (Dihydroxyphenylacetic Acid)
Dopamine
Homovanillic Acid

HPLC Separation of DOPAC, Homovanillic Acid and Dopamine on the Primesep 100 Column
  Application Notes: Neurotransmitters DOPAC, homovanillic acid and dopamine were separated by mixed-mode chromatography on Primesep 100 and Primesep 200 HPLC columns. The method can be used for quantification of neurotransmitters with LC/MS compatible conditions. The compounds are retained by combination of reversed-phase, ion-exchange or ion-exclusion mechanisms. The retention time and selectivity of separation can be adjusted by variation of amount of acetonitrile, buffer pH and buffer concentration.   Application Columns: Primesep 100, Primesep 200   Application compounds: Dopac, Homovanillic Acid, Dopamine Detection technique: UV, LC/MS, ELSD/CAD

Application Analytes:

DOPAC (Dihydroxyphenylacetic Acid)
Dopamine
Homovanillic Acid

HPLC Separation of DOPAC, Homovanillic Acid in Dopamine on the Primesep 200 Column

 

Application Notes: Neurotransmitters DOPAC, homovanillic acid and dopamine were separated by mixed-mode chromatography on Primesep 100 and Primesep 200 HPLC columns. The method can be used for quantification of neurotransmitters with LC/MS compatible conditions. The compounds are retained by combination of reversed-phase, ion-exchange or ion-exclusion mechanisms. The retention time and selectivity of separation can be adjusted by variation of amount of acetonitrile, buffer pH and buffer concentration.
Application Columns: Primesep 100, Primesep 200
Application compounds: Dopac, Homovanillic Acid, Dopamine
Detection technique: UV, LC/MS, ELSD/CAD



Application Analytes:

DOPAC (Dihydroxyphenylacetic Acid)
Dopamine
Homovanillic Acid

Generic Screening Method for Complex Mixtures



Application Analytes:

2,3-Dihydroxybenzoic Acid
2,6-Lutidine
Amitriptyline
Benzoic Acid
Benzylamine
DOPA (3,4-dihydroxy-L-phenylalanine)
Epinephrine
Ethyl Paraben
Homovanillic Acid
Hydroxytryptophan
Methyl Paraben
Phenol
Toluene
Tryptophan
Uracil