Methylmalonic Acid

Methylmalonic Acid structural formula

CAS Number516-05-2
Molecular FormulaC4H6O4
Molecular Weight118.088
InChI KeyZIYVHBGGAOATLY-UHFFFAOYSA-N
LogP-0.367
Synonyms
  • Methylmalonic acid
  • Methylpropanedioic acid
  • Propanedioic acid, 2-methyl-
  • 516-05-2
  • 4-02-00-01932
  • Propanedioic acid, methyl-
  • 1,1-Ethanedicarboxylic acid
  • 2-Methylmalonic acid
  • acide methylmalonique
  • acido metilmalonico
  • Isosuccinic acid
  • Malonic acid, methyl-
  • Methylmalonsaeure
  • Methylpropanedioic acid
  • NSC 25201
  • Propanedioic acid, 2-methyl-
  • BRN 1756084
  • EINECS 208-219-5
  • UNII-8LL8S712J7
  • 1,1-Ethanedicarboxylate
  • 2-Methylmalonate
  • 2-methylpropanedioic acid
  • Isosuccinate
  • Methyl-Malonate
  • Methyl-Malonic acid
  • Methyl-Propanedioate
  • Methyl-Propanedioic acid
  • Methylmalonate
  • Methylpropanedioate
  • alpha-methylmalonic acid

Applications:

HPLC Method for Analysis of Dibasic Acids on Newcrom B Column

July 29, 2022

Separation type: Liquid Chromatography Mixed-mode

 

PLC Method for Analysis of Dibasic acid on Newcrom B Column

Succinic acid is a key intermediary (in the form of its anion, succinate) in the electron transport chain, a process that is the key power source for our cells. It also has a few limited functions outside of the mitochondria. Glutaric acid is a common plasticizer precursor and often used in the production of polyester polyols and polyamides. Interestingly, it is significantly more water soluble than either Succinic or Adipic acid. Adipic acid, also known as hexanedioic acid, is a key precursor to nylon, and more than 2.5 billion kilograms are produced every year. Methylmalonic acid is a key intermediary in the anaplerotic reactions of the Krebs cycle. These Dibasic acids can be separated, retained, and analyzed on a mixed-mode Newcrom B column with a mobile phase consisting of water, Acetonitrile (MeCN), and Sulfuric acid (FA). This analytical method can be UV detected at 200 nm with high resolution and peak symmetry.

High Performance Liquid Chromatography (HPLC) Method for Analysis of Adipic Acid, Succinic Acid, Glutaric Acid, Methylmalonic acid

Condition

Column Newcrom B, 4.6×150 mm,  100A
Mobile Phase MeCN/H2O -5/95%
Buffer H2SO4 – 0.02%
Flow Rate 1.0 ml/min
Detection UV 200 nm

Description

Class of Compounds Acid, Hydrophilic
Analyzing Compounds Adipic Acid, Succinic Acid, Glutaric Acid, Methylmalonic acid

 

Application Column

Newcrom B

The Newcrom columns are a family of reverse-phase-based columns. Newcrom A, AH, B, and BH are all mixed-mode columns with either positive or negative ion-pairing groups attached to either short (25 Å) or long (100 Å) ligand chains. Newcrom R1 is a special reverse-phase column with low silanol activity.

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Application Analytes:
Adipic Acid
Glutaric acid
Methylmalonic Acid
Succinic Acid
SIELC Technologies usually develops more than one method for each compound. Therefore, this particular method may not be the best available method from our portfolio for your specific application. Before you decide to implement this method in your research, please send us an email to research@sielc.com so we can ensure you get optimal results for your compound/s of interest.

HPLC Separation of Methylmalonic and Malonic Acid on Newcrom B Column

November 20, 2019


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HPLC Separation of Methylmalonic and Malonic Acid on Newcrom B Column_1199

Condition

Column Newcrom B, 4.6×150 mm, 5 µm, 100A
Mobile Phase MeCN/H2O – 10/90%
Buffer H3PO4 – 0.02%
Flow Rate 1.0 ml/min
Detection UV 200nm

Description

Class of Compounds Acid, Hydrophilic, Polar
Analyzing Compounds Methylmalonic acid,  Malonic Acid

Application Column

Newcrom B

The Newcrom columns are a family of reverse-phase-based columns. Newcrom A, AH, B, and BH are all mixed-mode columns with either positive or negative ion-pairing groups attached to either short (25 Å) or long (100 Å) ligand chains. Newcrom R1 is a special reverse-phase column with low silanol activity.

Select options
Application Analytes:
Malonic Acid
Methylmalonic Acid
SIELC Technologies usually develops more than one method for each compound. Therefore, this particular method may not be the best available method from our portfolio for your specific application. Before you decide to implement this method in your research, please send us an email to research@sielc.com so we can ensure you get optimal results for your compound/s of interest.

HPLC Separation of Methylmalonic, Fumaric, Maleic acids on Newcrom B Column

October 28, 2019


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Condition

Column Newcrom B, 4.6×150 mm, 5 µm, 100A
Mobile Phase MeOH/H2O – 5/93%
Buffer Formic Acid – 2%
Flow Rate 1.0 ml/min
Detection CAD (Corona)  (MS-compatible mobile phase)

Description

Class of Compounds Acid, Hydrophilic, Ionizable
Analyzing Compounds Methylmalonic acid, Fumaric acid, Maleic acid

 

Application Column

Newcrom B

The Newcrom columns are a family of reverse-phase-based columns. Newcrom A, AH, B, and BH are all mixed-mode columns with either positive or negative ion-pairing groups attached to either short (25 Å) or long (100 Å) ligand chains. Newcrom R1 is a special reverse-phase column with low silanol activity.

Select options
Application Analytes:
Fumaric Acid
Maleic Acid
Methylmalonic Acid
SIELC Technologies usually develops more than one method for each compound. Therefore, this particular method may not be the best available method from our portfolio for your specific application. Before you decide to implement this method in your research, please send us an email to research@sielc.com so we can ensure you get optimal results for your compound/s of interest.

HPLC Separation of Small Organic Acids on Newcrom B Column

October 22, 2019


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Condition

Column Newcrom B, 4.6×150 mm, 5 µm, 100A
Mobile Phase MeCN/H2O – 5/95%
Buffer AmAc pH 5.0, Formic Acid
Flow Rate 1.0 ml/min
Detection CAD (Corona) MS- compatible mobile phase

Description

Class of Compounds Acid, Hydrophilic, Ionizable
Analyzing Compounds Glycolic acid, Succinic Acid, Methylmalonic acid, Malic Acid, Maleic Acid, Citric Acid, Tartaric Acid, Fumaric Acid, Malonic Acid

Application Column

Newcrom B

The Newcrom columns are a family of reverse-phase-based columns. Newcrom A, AH, B, and BH are all mixed-mode columns with either positive or negative ion-pairing groups attached to either short (25 Å) or long (100 Å) ligand chains. Newcrom R1 is a special reverse-phase column with low silanol activity.

Select options
Application Analytes:
Citric Acid
Fumaric Acid
Gluconic acid
Glycolic acid
Maleic Acid
Malic Acid
Malonic Acid
Methylmalonic Acid
Succinic Acid
Tartaric Acid
dl-Tartaric acid
SIELC Technologies usually develops more than one method for each compound. Therefore, this particular method may not be the best available method from our portfolio for your specific application. Before you decide to implement this method in your research, please send us an email to research@sielc.com so we can ensure you get optimal results for your compound/s of interest.

HPLC Separation of Succinic and Methylmalonic Acids

November 12, 2010


Organic and inorganic acids can be separated by reversed-phase anion-exchange Primesep D column. Succinic and methylmalonic acids were separated based on their hydrophobic and ionic properties. Method can be used for analysis of other acids by HPLC.

Application Column

Primesep D

The Primesep family of mixed-mode columns offers a wide variety of stationary phases, boasting unprecedented selectivity in the separation of a broad array of chemical compounds across multiple applications. Corresponding Primesep guard columns, available with all stationary phases, do not require holders. SIELC provides a method development service available to all customers. Inquire about our specially-tailored custom LC-phases for specific separations.

Select options
Application Analytes:
Methylmalonic Acid
Organic Acids
Succinic Acid

Application Detection:
ELSD Detection
SIELC Technologies usually develops more than one method for each compound. Therefore, this particular method may not be the best available method from our portfolio for your specific application. Before you decide to implement this method in your research, please send us an email to research@sielc.com so we can ensure you get optimal results for your compound/s of interest.

HILIC Separation of Carboxylic Acids

August 22, 2008

Hydrophilic acids are separated on Obelisc N mixed-mode HILIC column. Seven carboxylic acids are separated based on their polarity and pKa values. Changes in ionization states of acids and stationary phase can be used to control elution order of organic and inorganic acids.

Application Column

Obelisc N

SIELC has developed the Obelisc™ columns, which are mixed-mode and utilize Liquid Separation Cell technology (LiSC™). These cost-effective columns are the first of their kind to be commercially available and can replace multiple HPLC columns, including reversed-phase (RP), AQ-type reversed-phase, polar-embedded group RP columns, normal-phase, cation-exchange, anion-exchange, ion-exclusion, and HILIC (Hydrophilic Interaction Liquid Chromatography) columns. By controlling just three orthogonal method parameters - buffer concentration, buffer pH, and organic modifier concentration - users can adjust the column properties with pinpoint precision to separate complex mixtures.

Select options
Application Analytes:
Fumaric Acid
Hydroxybenzoic Acid
Malic Acid
Mandelic Acid
Methylmalonic Acid
Organic Acids
Succinic Acid
Tartaric Acid

Application Detection:
ELSD Detection
SIELC Technologies usually develops more than one method for each compound. Therefore, this particular method may not be the best available method from our portfolio for your specific application. Before you decide to implement this method in your research, please send us an email to research@sielc.com so we can ensure you get optimal results for your compound/s of interest.

HPLC Separation of Organic Acids in HILIC Mode on Primesep N Column

December 6, 2007

Ascorbic, methylmalonic and succinic are weak organic acids. Retention of these three acids is achieved on Primesep N column in HILIC mode using acetonitrile/water and ammonium acetate. Compounds are monitored by ELSD. Method can be used for determination of ascorbic acid (Vitamin C), methylmalonic acid and succinic acid in various matrices. Other polar organic acids can be analyzed on this HILIC column.

Condition

Column Primesep  N , 4.6×150 mm, 5 µm, 100A
Mobile Phase MeCN/H2O
Buffer AmAc
Flow Rate 1.0 ml/min
Detection ELSD

 

Description

Class of Compounds
Acid, Vitamin B₆, Hydrophobic, Ionizable
Analyzing Compounds Ascorbic acid (Vitamin C), Methylmalonic acid,  Succinic acid

Application Column

Primesep N

The Primesep family of mixed-mode columns offers a wide variety of stationary phases, boasting unprecedented selectivity in the separation of a broad array of chemical compounds across multiple applications. Corresponding Primesep guard columns, available with all stationary phases, do not require holders. SIELC provides a method development service available to all customers. Inquire about our specially-tailored custom LC-phases for specific separations.

Select options
Application Analytes:
Ascorbic Acid
Methylmalonic Acid
Organic Acids
Succinic Acid

Application Detection:
ELSD Detection
SIELC Technologies usually develops more than one method for each compound. Therefore, this particular method may not be the best available method from our portfolio for your specific application. Before you decide to implement this method in your research, please send us an email to research@sielc.com so we can ensure you get optimal results for your compound/s of interest.