| CAS Number | 60-87-7 |
|---|---|
| Molecular Formula | C17H20N2S |
| Molecular Weight | 284.421 |
| InChI Key | PWWVAXIEGOYWEE-UHFFFAOYSA-N |
| LogP | 4.81 |
| Synonyms |
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Applications:
Uv-Vis Spectrum of Promethazine
April 14, 2026
Access the UV-Vis Spectrum SIELC Library
If you are looking for optimized HPLC method to analyze Promethazine check our HPLC Applications library
For optimal results in HPLC analysis, it is recommended to measure absorbance at a wavelength that matches the absorption maximum of the compound(s) being analyzed. The UV spectrum shown can assist in selecting an appropriate wavelength for your analysis. Please note that certain mobile phases and buffers may block wavelengths below 230 nm, rendering absorbance measurement at these wavelengths ineffective. If detection below 230 nm is required, it is recommended to use acetonitrile and water as low UV-transparent mobile phases, with phosphoric acid and its salts, sulfuric acid, and TFA as buffers.
For some compounds, the UV-Vis Spectrum is affected by the pH of the mobile phase. This analysis was done with the mobile phase made of 20% acetonitrile and 80% water.
HPLC Analysis of Promethazine
July 31, 2015
Promethazine is a drug used as an antihistamine, a sedative, and used to reduce motion sickness, and prescribed for insomnia. The reverse-phase column Legacy L1 was used to retain promethazine. comparisons between Legacy L1 and Phenomenex columns are available upon request.
| Column | Legacy L1, 4.6×250 mm, 5 µm, 100A |
| Mobile Phase | MeCN – 35% |
| Buffer | NaH2PO4 pH 2.5 – 20 mM |
| Flow Rate | 1.0 ml/min |
| Detection | UV, 254 nm |
| Class of Compounds |
Drug, Hydrophobic, Ionizable |
| Analyzing Compounds | Promethazine |
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Application Column
Legacy L1
SIELC's family of Legacy columns is based on the United States Pharmacopeia's (USP) published chromatographic methods and procedures. Numerous brands have columns used in USP reference standards and methods. USP has created various designations to group together columns with similar types of packing and properties in the solid phase. SIELC's Legacy columns adhere to these strict requirements and properties, allowing you to easily replace older columns that are no longer available without needing to significantly modify your method or SOPs.
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