Access the UV-Vis Spectrum SIELC Library

If you are looking for optimized HPLC method to analyze Pyrimethanil check our HPLC Applications library

For optimal results in HPLC analysis, it is recommended to measure absorbance at a wavelength that matches the absorption maximum of the compound(s) being analyzed. The UV spectrum shown can assist in selecting an appropriate wavelength for your analysis. Please note that certain mobile phases and buffers may block wavelengths below 230 nm, rendering absorbance measurement at these wavelengths ineffective. If detection below 230 nm is required, it is recommended to use acetonitrile and water as low UV-transparent mobile phases, with phosphoric acid and its salts, sulfuric acid, and TFA as buffers.
For some compounds, the UV-Vis Spectrum is affected by the pH of the mobile phase. The spectra presented here are measured with an acidic mobile phase that has a pH of 3 or lower.

Even though teas are known for containing polyphenols such as catechins, flavonoids and theaflavins, SIELC mixed-mode columns can be used to analyze a matrix from a commercial tea and retain target compounds. In this method, green tea was spiked with 0.005mg/ml of pesticides previously found in teas. Primesep 200 was used for it’s reverse-phase and acidic ion-pairing groups. Target compounds were retained and resolved from the majority of the tea matrix peak.

Tea has been known to contain residue of pesticides and fungicides such as picoxystrobin and pyrimethanil. We used Primesep 200, a reversed-phase column with weakly acidic ion-pairing groups to separate picoxystrobin, pyrimethanil and atorvastatin. A 15 minute method used to demonstrate retention control when adjusting ratio of organic to aqueous in the mobile phase. Baseline retention with good resolution was achieved.