Streptomycin

Streptomycin structural formula

CAS Number57-92-1
Molecular FormulaC21H39N7O12
Molecular Weight581.581
InChI KeyUCSJYZPVAKXKNQ-HZYVHMACSA-N
LogP-3.72
Synonyms
  • Streptomycin A
  • N,N'-[(1R,2R,3S,4R,5R,6S)-4-({5-Deoxy-2-O-[2-deoxy-2-(methylamino)-alpha-L-glucopyranosyl]-3-C-formyl-alpha-L-lyxofuranosyl}oxy)-2,5,6-trihydroxycyclohexane-1,3-diyl]diguanidine
  • Guanidine, N,N'''-[(1R,2R,3S,4R,5R,6S)-4-[[5-deoxy-2-O-[2-deoxy-2-(methylamino)-alpha-L-glucopyranosyl]-3-C-formyl-alpha-L-lyxofuranosyl]oxy]-2,5,6-trihydroxy-1,3-cyclohexanediyl]bis-
  • 57-92-1
  • D-Streptamine, O-2-deoxy-2-(methylamino)-alpha-L-glucopyranosyl-(1->2)-O-5-deoxy-3-C-formyl-alpha-L-lyxofuranosyl-(1->4)-N,N'-bis(aminomethyl)-
  • 4-18-00-07540
  • D-Streptamine, O-2-deoxy-2-(methylamino)-α-L-glucopyranosyl-(1-2)-O-5-deoxy-3-C-formyl-α-L-lyxofuranosyl-(1-4)-N,N'-bis(aminoiminomethyl)-
  • 2,4-Diguanidino-3,5,6-trihydroxycyclohexyl 5-deoxy-2-O-(2-deoxy-2-methylamino-α-glucopyranosyl)-3-formylpentofuranoside
  • Agrimycin
  • D-Streptamine, O-2-deoxy-2-(methylamino)-α-L-glucopyranosyl-(1-2)-O-5-deoxy-3-C-formyl-α-L-lyxofuranosyl-(1-4)-N1,N3-bis(aminoiminomethyl)-
  • D-Streptamine,O-2-deoxy-2-(methylamino)-α-L-glucopyranosyl-(1-2)-O-5-deoxy-3-C-formyl-α-L-lyxofuranosyl-(1-4)-N,N'-bis(aminoiminomethyl)-
  • estreptomicina
  • Neodiestreptopab
  • NSC 14083
  • streptomycin
  • streptomycine
  • BRN 0074498
  • Caswell No. 804
  • 2,4-Diguanidino-3,5,6-trihydroxycyclohexyl 5-deoxy-2-O-(2-deoxy-2-methylamino-alpha-L-glucopyranosyl)-3-C-formyl-beta-L-lyxopentanofuranoside
  • EINECS 200-355-3
  • EPA Pesticide Chemical Code 006306
  • Hokko-mycin
  • Strepcen
  • Streptomycinum
  • Streptomicina
  • Streptomyzin
  • UNII-Y45QSO73OB
  • SM
  • [2-deoxy-2-(dimethylamino)-alpha-L-glucopyranosyl]-(1->2)-[5-deoxy-3-C-formyl-alpha-L-lyxofuranosyl]-(1->4)-{N',N'''-[(1,3,5/2,4,6)-2,4,5,6-tetrahydroxycyclohexane-1,3-diyl]diguanidine}
  • streomycin
  • 364062-67-9
  • 47814-83-5
  • 47816-81-9
  • 82958-69-8

Applications:

HPLC MS Method for Analysis of Streptomycin on Chromni Column

September 10, 2026

HPLC Method for Analysis of Streptomycin on Chromni Column by SIELC Technologies

High Performance Liquid Chromatography (HPLC) Method for Analysis of Streptomycin.

Streptomycin is the an antibiotic with the chemical formula C21H39N7O12. It is primarily used to treat bacterial infections but not limited to tuberculosis, endocarditis, brucellosis, and the plague. It is typically given intramuscularly. It works as a protein synthesis inhibitor by binding to the 16S rRNA of the 30S ribosomal subunit, which leads to it interfering with the binding of formyl-methionyl-tRNA. It is the official state microbe of New Jersey. You can find detailed UV spectra of Streptomycin and information about its various lambda maxima by visiting the following link.

Streptomycin can be retained and analyzed using the Chromni stationary phase column. The analysis utilizes a gradient method with a simple mobile phase consisting of water and acetonitrile (MeCN). Detection is performed using LC MS.

Condition

ColumnChromni, 3.2 x 100 mm, 3 µm, 100 A, dual ended
Mobile PhaseMeCN/H2O
BufferAmmonium Formate
Flow Rate0.5 ml/min
DetectionLC MS ESI SIM 582 (M+H)+
LOD*0.2 ppm
*LOD was determined for this combination of instrument, method, and analyte, and it can vary from one laboratory to another even when the same general type of analysis is being performed.

Description

Class of CompoundsAntibiotic
Analyzing CompoundsStreptomycin

Application Column

Chromni

Column Diameter: 3.2 mm
Column Length: 100 mm
Particle Size: 3 µm
Pore Size: 100 A
Column options: dual ended

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Application Analytes:
Streptomycin
Application Detection:
LC MS Detection
SIELC Technologies usually develops more than one method for each compound. Therefore, this particular method may not be the best available method from our portfolio for your specific application. Before you decide to implement this method in your research, please send us an email to research@sielc.com so we can ensure you get optimal results for your compound/s of interest.

Uv-Vis Spectrum of Streptomycin

January 19, 2026
Access the UV-Vis Spectrum SIELC Library
UV-Vis Spectrum of Streptomycin.

If you are looking for optimized HPLC method to analyze Streptomycin check our HPLC Applications library

For optimal results in HPLC analysis, it is recommended to measure absorbance at a wavelength that matches the absorption maximum of the compound(s) being analyzed. The UV spectrum shown can assist in selecting an appropriate wavelength for your analysis. Please note that certain mobile phases and buffers may block wavelengths below 230 nm, rendering absorbance measurement at these wavelengths ineffective. If detection below 230 nm is required, it is recommended to use acetonitrile and water as low UV-transparent mobile phases, with phosphoric acid and its salts, sulfuric acid, and TFA as buffers.
For some compounds, the UV-Vis Spectrum is affected by the pH of the mobile phase. The spectra presented here are measured with an acidic mobile phase that has a pH of 3 or lower.

 

Application Analytes:
Streptomycin
SIELC Technologies usually develops more than one method for each compound. Therefore, this particular method may not be the best available method from our portfolio for your specific application. Before you decide to implement this method in your research, please send us an email to research@sielc.com so we can ensure you get optimal results for your compound/s of interest.

HPLC Determination of Streptomycin on Primesep 200 Column

May 9, 2019

HPLC Method for Streptothricins, Streptomycin B, Streptomycin on Primesep 200 by SIELC Technologies

HPLC Determination of Streptomycin sulfate salt on Primesep 200 Column

High Performance Liquid Chromatography (HPLC) Method for Analysis of Streptothricins, Streptomycin B, Streptomycin.

Streptomycin is an antibiotic used in treating bacterial infections and has also been used as a pesticide. It is on the World Health Organization’s (WHO) list of essential medicines. It works by inhibiting the synthesis of proteins. It can be retained on a Primesep 200 mixed-mode column by hydrophobic and ion-exchange properties. The analytical method uses MS-compatible mobile phase of acetonitrile (ACN) and water with sulfuric acid (H2SO4) buffer. Also capable of UV detection at 200nm.

Condition
Column Primesep 200
Mobile Phase MeCN/H2O – 10/90%
Buffer H2SO4 – 0.05%
Flow Rate 1.0 ml/min
Detection UV, 200 nm

 

Description
Class of Compounds
 Drug, Hydrophilic, Zwitterionic, Antibiotic
Analyzing Compounds Streptothricins, Streptomycin B, Streptomycin

 

Application Column

Primesep 200

Column Diameter: 4.6 mm
Column Length: 150 mm
Particle Size: 5 µm
Pore Size: 100 A
Column options: dual ended

Add to cart
Application Analytes:
Streptomycin
Streptomycin B
Streptothricins
Application Detection:
UV Detection
SIELC Technologies usually develops more than one method for each compound. Therefore, this particular method may not be the best available method from our portfolio for your specific application. Before you decide to implement this method in your research, please send us an email to research@sielc.com so we can ensure you get optimal results for your compound/s of interest.