Vitamin B3 (Niacin)

Vitamin B3 (Niacin) structural formula

CAS Number59-67-6
Molecular FormulaC6H5NO2
Molecular Weight123.112
InChI KeyPVNIIMVLHYAWGP-UHFFFAOYSA-N
LogP0.36
Synonyms
  • Nicotinic acid
  • Pyridine-3-carboxylic acid
  • 3-Pyridinecarboxylic acid
  • 59-67-6
  • Niacin
  • 5-22-02-00057
  • 3-Pyridinecarboxylic acid
  • 3-Carboxylpyridine
  • 3-Carboxypyridine
  • 3-Pyridylcarboxylic acid
  • Acide nicotinique
  • acido nicotinico
  • Apelagrin
  • Enduracin
  • Niaspan
  • Nicacid
  • Nicangin
  • Nicobid
  • Nicodelmine
  • Nicolar
  • Niconacid
  • Nico-Span
  • Nicotinipca
  • NICOTINSAEURE
  • Nicotinsaure
  • NSC 169454
  • Pellagrin
  • Pelonin
  • Slo-niacin
  • Wampocap
  • β-Pyridinecarboxylic acid
  • Acidum nicotinicum
  • BRN 0109591
  • Caswell No. 598
  • Davitamon PP
  • Direktan
  • EINECS 200-441-0
  • EPA Pesticide Chemical Code 056701
  • Nicagin
  • Nicocidin
  • Nicocrisina
  • Niconat
  • Niconazid
  • Nicosyl
  • Nicotamin
  • Nicotene
  • Nicotil
  • Nicotine acid
  • Nicovasan
  • Nicovasen
  • Nipellen
  • Pellagramin
  • Pyridine-beta-carboxylic acid
  • SK-Niacin
  • Tega-Span
  • Vitaplex N
  • Niaspan Titration Starter Pack
  • Kyselina nikotinova
  • Pyridine-carboxylique-3
  • UNII-2679MF687A
  • 3-Picolinic acid
  • 3-Pyridinecarboxylate
  • 3-Pyridylcarboxylate
  • Akotin
  • Daskil
  • Efacin
  • Induracin
  • Linic
  • Niac
  • Niacine
  • Niacor
  • Nicamin
  • Nicocap
  • Nicosan 3
  • Nicotinate
  • Nicyl
  • Nikotinsaeure
  • Nyclin
  • P.P. factor
  • PP factor
  • Pellagrin
  • Peviton
  • Tinic
  • anti-pellagra vitamin
  • beta-pyridinecarboxylic acid
  • m-pyridinecarboxylic acid
  • pellagra preventive factor
  • pyridine-3-carboxylic acid
  • pyridine-beta-carboxylic acid
  • vitamin B3
  • 123574-58-3

Applications:

USP Methods for the Analysis of Niacin with the Legacy L1 Column

June 21, 2012

Niacin (vitamin B3, nicotinic acid) is one of the essential vitamins. The water-soluble vitamin was analyzed by reversed-phase chromatography on the C18 reversed-phase HPLC column, Legacy L1. This column is classified according to USP as a L1-type of column. It can be used for the analysis of various drugs, vitamins, and chemicals according to United States Pharmacopeia.

Condition

Column Legacy L1, 4.6×150 mm, 5 µm, 100A
Mobile Phase Hexanesulfonate/MeOH/MeCN/AcOH –78/14/7/2
Buffer Hexanesulfonate
Flow Rate 1.0 ml/min
Detection UV, 250 nm

 

Description

Class of Compounds
Drug, Cholesterol medication, Vitamin B3, Hydrophobic, Ionizable
Analyzing Compounds Vitamin B3, Niacin

 

Application Column

SIELC Legacy L1 HPLC column

Legacy L1

SIELC's family of Legacy columns is based on the United States Pharmacopeia's (USP) published chromatographic methods and procedures. Numerous brands have columns used in USP reference standards and methods. USP has created various designations to group together columns with similar types of packing and properties in the solid phase. SIELC's Legacy columns adhere to these strict requirements and properties, allowing you to easily replace older columns that are no longer available without needing to significantly modify your method or SOPs.

Select options
Application Analytes:
Vitamin B3 (Niacin)
SIELC Technologies usually develops more than one method for each compound. Therefore, this particular method may not be the best available method from our portfolio for your specific application. Before you decide to implement this method in your research, please send us an email to research@sielc.com so we can ensure you get optimal results for your compound/s of interest.

HILIC Separation of Vitamins Group B

August 22, 2008

Separation of vitamins group B is achieved on Obelisc N column in HILIC mixed-mode. Vitamins of this group are different in polarity and ionic properties. Retention and separation is achieved by optimization of amount of ACN, buffer and buffer pH. Combination of UV and ELSD detection is used to monitor HPLC separation. B vitamins are water-soluble vitamins that play an important role in cell metabolism. Supplements containing all six are generally referred to as a vitamin B complex. Individual B vitamin supplements are referred to by the specific name of each vitamin. This method can be used to analyze individual B vitamins as well as vitamin B complex. Isolation of impurities as well as degradation products is possible by preparative chromatography.

Condition

Column Obelisc N , 4.6×150 mm, 5 µm, 100A
Mobile Phase MeCN/H2O
Buffer AmAC pH 4.5 – 10 mM
Flow Rate 1.0 ml/min
Detection UV, 270 nm, ELSD

 

Description

Class of Compounds
Drug, Vitamin B₆, Hydrophobic, Ionizable
Analyzing Compounds Pyridoxine,  Niacinamide, Pantothenic acid, Riboflavin

Application Column

Obelisc N

SIELC has developed the Obelisc™ columns, which are mixed-mode and utilize Liquid Separation Cell technology (LiSC™). These cost-effective columns are the first of their kind to be commercially available and can replace multiple HPLC columns, including reversed-phase (RP), AQ-type reversed-phase, polar-embedded group RP columns, normal-phase, cation-exchange, anion-exchange, ion-exclusion, and HILIC (Hydrophilic Interaction Liquid Chromatography) columns. By controlling just three orthogonal method parameters - buffer concentration, buffer pH, and organic modifier concentration - users can adjust the column properties with pinpoint precision to separate complex mixtures.

Select options
Application Analytes:
Niacinamide
Vitamin B1 (Thiamine)
Vitamin B2 (Riboflavin)
Vitamin B3 (Niacin)
Vitamin B5 (Pantothenic Acid)
Vitamin B6 (Pyridoxine)

Application Detection:
ELSD Detection
UV Detection
SIELC Technologies usually develops more than one method for each compound. Therefore, this particular method may not be the best available method from our portfolio for your specific application. Before you decide to implement this method in your research, please send us an email to research@sielc.com so we can ensure you get optimal results for your compound/s of interest.