|
Compound: Benzoquinone
Dewetting Study in Aqueous Conditions |
 |
Application Notes: Most reversed-phase HPLC columns, such as C18, are incompatible with 100% aqueous mobile phases due to dewetting (collapse) of the stationary phase. Primesep columns combine reversed-phase with polar functional groups which eliminate the problems of dewetting. The Primesep 100 column in this application with an acetonitrile (MeCN, ACN) shows a retention time of 2.5 minutes. In 100% water, the retention time is 3 minutes even after sitting 24 hours in the water mobile phase. On a C18 phase, the analyte retention would have been lost and elution at the void (1.5 minutes) would have resulted. Application Analytes: Application Detection: |
SIELC Technologies | 65 East Palatine Road, Suite 221, Prospect Heights, IL USA 60070 | Tel: (847) 229-2629 Fax: (847) 655-6079 | Email: mail@sielc.com | sitemap
Copyright © SIELC Technologies. 2002-2007
Copyright © SIELC Technologies. 2002-2007