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Separation of Zn and Mn on Primesep 100 Column |
 | Application Notes:
Primesep 100 separates the divalent cations, zinc and manganese, from their counter ion, chloride. The cations are retained by cation exchange and the chloride anion is eluted by ion exclusion. The mobile phase mixture of water, acetonitrile (MeCN, ACN) and ammonium acetate is evaporative light scattering detection (ELSD) compatible.
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Separation of Metal Ions on Primesep 100 Column |
 | Application Notes:
Primesep 100 separates the monovalent cations, lithium, potassium, and sodium, and the divalent cations, zinc, manganese, and calcium. The cations are resolved by cation exchange. The mobile phase mixture of water, acetonitrile (MeCN, ACN) and trifluoroacetic acid (TFA) is evaporative light scattering detection (ELSD) compatible.
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Zn and Na Quantitation in Drug Substances |
 | Application Notes:
Primesep 100 allows the quantitation of sodium and zinc cations in a drug product sample. The cations are retained by cation exchange and the drug product is retained by hydrophobic, reversed-phase mechanisms. The separation uses a mobile phase mixture of water, acetonitrile (MeCN, ACN) and trifluoroacetic acid (TFA) with evaporative light scattering detection (ELSD) and UV detection.
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Simultaneous Separation of Cations and Anions |
 | Application Notes:
Primesep 100 and Primesep B columns connected in series allow the quantitation of sodium chloride and potassium bromide ions in one injection. The Primesep B column retains the chloride and bromide anions by anion exchange, and the Primesep 100 retains the sodium and potassium captions by cation exchange. The separation uses a mobile phase mixture of water, acetonitrile (MeCN, ACN) and trifluoroacetic acid (TFA) with evaporative light scattering detection (ELSD).
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Effect of Concentration of Organic Modifier on Retention of Cation and Anions |
 | Application Notes:
Primesep 100 and Primesep B columns connected in series allow the quantitation of sodium chloride and potassium bromide ions in one injection. The Primesep B column retains the chloride and bromide anions by anion exchange, and the Primesep 100 retains the sodium and potassium cations by cation exchange. The retention can be adjusted by changing the water/acetonitrile ratio in the mobile phase. The separation uses a mobile phase mixture of water, acetonitrile (MeCN, ACN) and ammonium acetate with evaporative light scattering detection (ELSD).
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HPLC Separation of Chloride and Nitrate Ions |
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HPLC Separation of Sodium, Chloride, Bromide, and Iodine |
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Comparison of Obelisc N, SeQuant® HILIC, and Bare Silica |
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HPLC Separation of Potassium, Perchlorate, Methanesulfonic, Chloride, Bromide, and Nitrate Ions on Obelisc N |
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