Compound: Deoxybenzoin

Reverse Mode vs Normal Mode: Separation of Aromatic Compounds

Application Notes:

Primesep 100 separates a mixture of benzyl compounds in both normal- and reversed-phase modes with UV detection. The peak order of benzil, benzoin, bibenzylbenzoate, bibenzyl, deoxybenzoin, and 4-methyldeoxybenzoin are completely reversed with a change of mobile phase. The reversed-phase mobile phase of water, acetonitrile (MeCN, ACN) and trifluoroacetic acid (TFA) and the normal phase mobile phase of hexane and isopropanol both separate this mixture in less than 12 minutes total elution time.

Application Columns:

Primesep^® 100 is a mixed-mode HPLC column with general hydrophobic and ion-exchange properties. The Primesep 100 stationary phase combines a hydrophobic chain and an acidic functional group as a cation exchanger. Acids, bases, zwitterions and neutrals can be retained and separated by Primesep 100 columns. Primesep 100 retains hydrophobic compounds by reversed-phase retention alone or a combination of reversed-phase and ion-exchange retention. Primesep 100 separates underivatized amino acids and amines by combining reversed-phase and ion-exchange mechanisms. In this case the acidic functional group acts as an embedded ion-pairing reagent. Organic acids are separated by reversed phase and ion exclusion. Inorganic cations can be separated by cation exchange. Methods can be developed on Primesep columns that are fully compatible with detectors such as UV, RI, conductivity, fluorescence, evaporative light scattering (ELSD) as well as mass spectrometers (LC/MS) and preparative chromatography (prep HPLC) systems.

Application Analytes:

Application Detection:

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