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Compound: Guanidine
Separation of Guanidine |
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Application Notes: Primesep 100 separates guanidine from its sodium counter ion on a short 50 mm column by cation exchange. Guanidine is found in urine as a product of protein metabolism and is also used in the manufacture of plastics and explosives. Guanidine is detected by UV detection at 200 nm, but UV cannot detect sodium ion. If an evaporative light scattering detector (ELSD) is used, both components can be detected. The separation method uses a mobile phase mixture of water, acetonitrile (MeCN, ACN) and trifluoracetic acid (TFA). Application Analytes: Application Detection: |
HPLC Application for Retention of Guanidine by Mixed-Mode Chromatography |
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Application Notes: Guanidine is highly basic (pKa 12.5) and polar compound formed by oxidation of guanine. In human body guanine is a by-product of protein metabolism and can be found in urine. Guanine can be retained by cation-exchange mechanism on Primesep 100 column. Guanine is slightly UV active and can be observed at 200 nm. A better detection technique is ELSD, which allows you to see low concentrations of guanine. Temperature of ELSD must be maintained relatively low to insure proper sensitivity of the method. This method can be used for analysis of other guanidine-based compounds. If substituted guanidine has hydrophobic properties it will retain based on ion-exchange and reverse phase mechanisms. Application Analytes: Application Detection: |
HPLC Separation of Guanidine and Benzoic Acids on Primesep 100 Column |
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Application Notes: Application Analytes: Application Detection: |
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Copyright © SIELC Technologies. 2002-2007
Copyright © SIELC Technologies. 2002-2007