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Compound: Phenylalanine
Amino Acids Analysis in Acid Gradient Condition |
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Application Notes: 11 underivatized amino acids (aspartic acid, glutamic acid, alanine, valine, methionine, isoleucine, cysteine, phenylalanine, histidine, lysine, and arginine) are separated by a Primesep 100 HPLC column by reversed-phase and ion-exchange mechanisms with LC/MS compatible conditions without the use of ion-pair reagents. The HPLC separation uses a TFA (trifluoroacetic acid) gradient in a mobile phase of water acetonitrile (MeCN, ACN with evaporative light scattering detection (ELSD). Application Analytes:
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Complex Mixture of Acids, Bases, Amino Acids, and Neutral Compounds |
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Application Notes: Primesep 100 separates a mixture of amino acids (tyrosine, phenylalanine), organic acids (benzoic acid, mandelic acid), amines (benzylamine, pyridine), and neutrals (benzonitrile, toluene) in one HPLC run by combining reversed-phase, cation-exchange, and polar interactions. The method is tunable and peak order can be changed significantly by adjusting acetonitrile and trifluoroacetic acid concentrations. The separation method uses a mobile phase mixture of water, acetonitrile (MeCN, ACN) and trifluoracetic acid (TFA) and compatible with UV, mass spec (LC/MS) and evaporative light scattering (ELSD) detection. Application Analytes: Application Detection: |
Catecholamine Pathway |
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Application Notes: Primesep 200 separates catecholamines in the catecholamine pathway in 10 minutes. Phenylalanine, tyrosine, DOPA, dopamine, norepinephrine, and epinephrine are baseline resolved by a combination of reversed-phase, ion-exchange, and ion-exclusion mechanisms. Excellent peak shape results with a mass spec compatible mobile phase of water, acetonitrile (MeCN, ACN) and trifluoracetic acid (TFA) with UV detection at 210 nm. Application Analytes: Application Detection: |
Bufferless Ion Separation (BLIS™) Chromatography of Amino Acids (1) |
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HPLC Separation of Acidic, Basic and Zwitterionic Compounds on Special Columns for Retention of Polar Compounds |
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HPLC Separation of Compounds of Catecholamine Pathway |
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Application Notes: The catecholamine neurotransmitters are amino acid derivatives of tyrosine. DOPA, tyrosine, phenylalanine, norepinephrine, epinephrine, and dopamine and baseline resolved on a Primesep 200 column. The HPLC separation uses a mobile phase of water, acetonitrile (MeCN, ACN), trifluoracetic acid (TFA) or ammonium formate and ultraviolet (UV) detection at 210 nm. Peak order and retention time can be changed by switching from TFA to ammonium formate in the mobile phase. Application Analytes: Application Detection: |
HPLC Separation of Amino Acids, Bases, Acids, and Neutrals on Obelisc R |
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Effect of both pH and Organic Content on a Separation of Sugars, Amino Acids, and Carboxylic Acids |
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HPLC Separation of Amino Acids on Obelisc R Column |
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HPLC Separation of Phenylalanine, Glucosamine, and Tryptophan on Mixed-Mode Column |
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Copyright © SIELC Technologies. 2002-2010
Copyright © SIELC Technologies. 2002-2010