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Compound: Sodium
Reverse Elution Order in Separation of Alkali Cation |
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Application Notes: Primesep C separates a mixture of inorganic cations, potassium, sodium, and lithium, by a combination of cation exchange and complex formation. The peak order is reversed on the Primesep C column compared to the order normally obtained on columns that rely on cation exchange alone. The mobile phase mixture of water, acetonitrile (MeCN, ACN) and ammonium acetate is compatible with UV and evaporative light scattering detection (ELSD). Application Analytes: Application Detection: |
Separation of Guanidine |
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Application Notes: Primesep 100 separates guanidine from its sodium counter ion on a short 50 mm column by cation exchange. Guanidine is found in urine as a product of protein metabolism and is also used in the manufacture of plastics and explosives. Guanidine is detected by UV detection at 200 nm, but UV cannot detect sodium ion. If an evaporative light scattering detector (ELSD) is used, both components can be detected. The separation method uses a mobile phase mixture of water, acetonitrile (MeCN, ACN) and trifluoracetic acid (TFA). Application Analytes: Application Detection: |
Complex of Cations on Primesep C is Effected by Organic Modifier |
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Application Notes: Primesep C separates a mixture of inorganic cations, potassium, sodium, and lithium, by a combination of cation exchange and complex formation. The peak order is reversed on the Primesep C column compared to the order normally obtained on columns that rely on cation exchange alone. In addition, the elution order can be rearranged by simply changing the organic modifier in the mobile phase from acetonitrile to methanol. The mobile phase mixture of water, acetonitrile (MeCN, ACN) or methanol (MeOH) and ammonium acetate is compatible with UV and evaporative light scattering detection (ELSD). Application Analytes: Application Detection: |
Separation of Metal Ions on Primesep 100 Column |
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Application Notes: Primesep 100 separates the monovalent cations, lithium, potassium, and sodium, and the divalent cations, zinc, manganese, and calcium. The cations are resolved by cation exchange. The mobile phase mixture of water, acetonitrile (MeCN, ACN) and trifluoroacetic acid (TFA) is evaporative light scattering detection (ELSD) compatible. Application Analytes: Application Detection: |
Zn and Na Quantitation in Drug Substances |
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Application Notes: Primesep 100 allows the quantitation of sodium and zinc cations in a drug product sample. The cations are retained by cation exchange and the drug product is retained by hydrophobic, reversed-phase mechanisms. The separation uses a mobile phase mixture of water, acetonitrile (MeCN, ACN) and trifluoroacetic acid (TFA) with evaporative light scattering detection (ELSD) and UV detection. Application Analytes: Application Detection: |
Simultaneous Separation of Cations and Anions |
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Application Notes: Primesep 100 and Primesep B columns connected in series allow the quantitation of sodium chloride and potassium bromide ions in one injection. The Primesep B column retains the chloride and bromide anions by anion exchange, and the Primesep 100 retains the sodium and potassium captions by cation exchange. The separation uses a mobile phase mixture of water, acetonitrile (MeCN, ACN) and trifluoroacetic acid (TFA) with evaporative light scattering detection (ELSD). Application Analytes: Application Detection: |
Effect of Concentration of Organic Modifier on Retention of Cation and Anions |
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Application Notes: Primesep 100 and Primesep B columns connected in series allow the quantitation of sodium chloride and potassium bromide ions in one injection. The Primesep B column retains the chloride and bromide anions by anion exchange, and the Primesep 100 retains the sodium and potassium cations by cation exchange. The retention can be adjusted by changing the water/acetonitrile ratio in the mobile phase. The separation uses a mobile phase mixture of water, acetonitrile (MeCN, ACN) and ammonium acetate with evaporative light scattering detection (ELSD). Application Analytes: Application Detection: |
Separation of Sodium, Potassium, Lithium, and Ammonium in Mixed-Mode HPLC |
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HPLC Separation of Sulfate and Phosphate Ions on Mixed-Mode HPLC |
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Application Notes: Sulfate and phosphate are separated on Primesep B2 and Primesep D column by anion-exchange mechanism. Because both sulfate and phosphate ions are not UV-active, ELSD is used to monitor separation of both anions. Method can be used to quantitate phosphate and sulfate in various pharmaceutical and chemical formulation, products and solution, drinking and ground water. Retention time is adjusted by increase or decrease of buffer concentrations. Two anions can be retained and separated on a very short column due to strong ion-exchange interaction. Method shows good reproducibility and versatility. Application Analytes: Application Detection: |
HPLC Separation of Citrulline and Arginine |
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HPLC Separation of Alkyl Sulfonic Acids |
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HPLC Separation of Surfactants |
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HPLC Separation of Sodium, Chloride, Bromide, and Iodine |
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HPLC Separation of Sugar Phosphates |
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SIELC Technologies | 65 East Palatine Road, Suite 221, Prospect Heights, IL USA 60070 | Tel: (847) 229-2629 Fax: (847) 655-6079 | Email: mail@sielc.com | sitemap
Copyright © SIELC Technologies. 2002-2007
Copyright © SIELC Technologies. 2002-2007