Compound: Sulfanilamide

Separation of Sulfonamides and Phenylhydrazine by Mixed-Mode HPLC

Application Notes:

Primesep 100 separates a mixture of sulfonamides (benzenesulfonamide, chlorobenzenesulfonamide, hydroxybenzenesulfonamide) and hydroxysulphaminophenylhydrazine by a mixture of polar and hydrophobic interactions. The stationary phase’s hydrophobic functionality provides a reversed-phase mechanism while the embedded cation-exchange group provides polar interactions. The separation method uses a mobile phase mixture of water, acetonitrile (MeCN, ACN) and trifluoracetic acid (TFA) with UV detection at 210 nm. This method is mass spec (LC/MS) and evaporative light scattering (ELSD) compatible.

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HPLC Separation of Aromatic Sulfonamides and Hydrozine

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HPLC Separation of Benzenesulfonamide and Sulfanilamide

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