Compound: Theobromine

Effect of Acid on Separation of Xanthines

Application Notes:

Primesep B2 separates xanthines such as theobromine, dimethylxanthine, theophylline by a combination of reversed-phase and ion-exchange mechanisms. The anion-exchange properties of the column allow retention of hydrophilic xanthines, and the reversed-phase properties retain hydrophobic compounds. The HPLC separation uses a mobile phase of water, acetonitrile (MeCN, ACN) and either trifluoroacetic acid (TFA), perchloric acid (HClO4), or sulfuric acid (H2SO4) and UV detection.

Application Analytes:

1,7-Dimethylxanthine
Theobromine
Theophylline

Application Detection:

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