Compound: Serotonin

Neurotransmitters

Application Notes:

The neurotransmitters dl-DOPA, creatinine, hydroxytryptophan (5-HTP), and serotonin are separated in less than 4 minutes on a short 50 mm Primesep 200 column. The HPLC separation uses a mobile phase of water, acetonitrile (MeCN, ACN) and trifluoroacetic acid (TFA) and UV detection at 250 nm. Ion-pair reagents were not needed for retention of these polar, hydrophilic compounds, instead a combination of ion-exchange and reversed-phase interactions were used.

Application Analytes:

Application Detection:

HPLC Separation of Neurotransmitters

Application Notes:

Dopamine, norphenylephrine and serotonin are separated on Primesep P HPLC column. Base line separation of these neurotransmitters can be achieved using mix-mode approach. Primesep P provides retention by reverse phase, pi-pi and cation-exchange mechanisms. Method can be used for fast quantitation of these compounds in various sample matrices. Detection technique ranges from UV to ELSD, LC/MS, RI and CAD. Primesep P column can be used with tetrahydrofuran or with 100% aqueous mobile phase to enhance pi-pi interaction.

Application Analytes:

Application Detection:

Separation of Serotonin, Dopamine, and Related Compounds

Application Notes:

Catecholamines are chemical compounds derived from the amino acid tyrosine containing catechol and amine groups. Some of them are biogenic amines. Retention of compounds of catecholamine pathway is achieved on Obelisc N column. All polar compounds are well retained by combination of HILIC and ion-exchange mechanisms. Obelisc N columns produce very good peak shapes for all analytes. Method is very sensitive to amount of ACN, buffer and buffer pH. Retention time changes with variation of main parameters. This method can be used for quantitation of biogenic amines and related compounds (homovanillic acid, dihydroxyphenyl acetic acid, serotonin, dopamine, epinephrine, hydroxytryptophan, epinephrine and DOPA) in urine, blood and other biological fluids. Further optimization of this HPLC method can be used during screening and validation. Amines and acids can be analyzed in the same run and retained by combination of polar organic mode, cation-exchange and anion-exchange modes. Various buffers within specified pH can be employed (ammonium formate, ammonium acetate, sodium phosphate, etc.).

Application Analytes:

Application Detection:

SIELC Technologies | 65 East Palatine Road, Suite 221, Prospect Heights, IL USA 60070 | Tel: (847) 229-2629 Fax: (847) 655-6079 | Email: mail@sielc.com | sitemap
Copyright © SIELC Technologies. 2002-2007