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Compound: Riboflavin (Vitamin B2)

 
   
 

Separation of Vitamin B


Riboflavin (Vitamin B2)

Application Notes:

Primesep C separates B vitamins with baseline resolution by a combination of cation exchange, complex formation, and hydrophobic interactions. Vitamin B1 (thiamine), vitamin B2 (riboflavin), and vitamin B6 (pyridoxine) are separated with a mobile phase of water, acetonitrile (MeCN, ACN) and triethylamine (TEA) phosphate with UV detection at 280 nm.

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Separation of Two Vitamins: Different Polarity - Isocratic Methods


Riboflavin (Vitamin B2)

Application Notes:

Primesep B and B2 columns separate Vitamins C (ascorbic acid) and B2 (riboflavin) with tunable selectivity. Peak order reversal is exhibited on these two columns with the same mobile phases due to their different polarity. The HPLC separation uses a mobile phase of water, acetonitrile (MeCN, ACN) and either formic or acetic acid (HOAc) with UV detection at 250 nm.

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HPLC Analysis of Active Drug in a Formulation


Riboflavin (Vitamin B2)

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